CAJ | 학술논문

目的明确内毒素脂多糖(LPS)对microRNA-142-3p(miR-142-3p)的表达调控作用及miR-142-3p的作用靶点.方法体外培养人单核细胞株THP-1,用LPS刺激48 h后,real-time RT-PCR方法检测miR-142-3p和腺苷酸环化酶9(AC-9)的表达变化.接着采用脂质体转染的方法,将miR-142-3p的拟似物(mimic)和抑制剂(inhibitor)转染进入THP-1细胞,观察AC-9的表达变化.应用SPSS13.0统计软件,采取t检验进行数据统计.结果 THP-1细胞受LPS刺激48 h后,miR-142-3p的表达上调(P＜0.05),AC-9表达则减少(P＜0.05).上调miR-142-3p水平后,细胞内AC-9的表达水平出现下降(P＜0.05),而抑制miR-142-3p的水平后,细胞内AC-9的表达水平上升(P＜0.05).结论 miR-142-3p可调控THP-1细胞AC-9基因表达水平,且可能具有类似LPS的促炎机制,提示miR-142-3p在调控免疫细胞的炎症反应过程中具有重要的作用.
목적 명학내독소지다당(LPS)대microRNA-142-3p(miR-142-3p)적표체조공작용급miR-142-3p적작용파점.방법 체외배양인단핵세포주THP-1,용LPS자격48 h후,real-time RT-PCR방법검측miR-142-3p화선감산배화매9(AC-9)적표체변화.접착채용지질체전염적방법,장miR-142-3p적의사물(mimic)화억제제(inhibitor)전염진입THP-1세포,관찰AC-9적표체변화.응용SPSS13.0통계연건,채취t검험진행수거통계.결과 THP-1세포수LPS자격48 h후,miR-142-3p적표체상조(P＜0.05),AC-9표체칙감소(P＜0.05).상조miR-142-3p수평후,세포내AC-9적표체수평출현하강(P＜0.05),이억제miR-142-3p적수평후,세포내AC-9적표체수평상승(P＜0.05).결론 miR-142-3p가조공THP-1세포AC-9기인표체수평,차가능구유유사LPS적촉염궤제,제시miR-142-3p재조공면역세포적염증반응과정중구유중요적작용.
Objective To investigate the regulatory effect of lipopolysaccharides (LPS) with regard to the expression of miR-142-3p and identify the target of miR-142-3p in THP-1 cells.Methods In our study,human monocytes THP-1 cell line was stimulated for 48 hours with 0.5 μ g/ml of LPS,transfected with miR-142-3p mimic and inhibitor (100 nM) by lipofectamine RNAiMAX.The levels of miR-142-3p and AC-9 mRNA in the THP-1 cells were detected by real-time RT-PCR.Statistical analysis was carried out by using SPSS 13.0 software,t-test was used.Results The level of AC-9 mRNA decreased and the level of miR-142-3p increased compared with that of the control group after 0.5 μ g/ml LPS stimulated.After 100 nM miR-142-3p mimic transfection,AC-9 mRNA level in THP-1 cells reduced,and after miR-142-3p inhibitor transfection,AC-9 mRNA level increased.Conclusion miR-142-3p may regulate THP-1 cells'inflammation response liked LPS by adjusting the AC-9 gene expression.miR-142-3p may play an important role in the inflammatory response of immune cells.