CAJ | 학술논문

目的:观察甲状旁腺激素相关蛋白(parathyroid hormone-related protein,PTHrP)对去卵巢大鼠牙槽骨的影响.方法:选用80只5月龄健康雌性大鼠,随机选其中60只行双侧卵巢摘除术(ovariectomized,OVX),另外20只行假手术不去卵巢;6周后,60只去除卵巢的大鼠再随机分为3组:PTHrP组注射PTHrP,雌激素组(E2组)注射苯甲酸雌二醇,安慰剂组(OVX组)注射生理盐水,每组20只;假手术的大鼠20只作为空白对照组(sham-OVX组)亦注射生理盐水.给药60 d后,处死大鼠解剖分离右侧下颌骨,测定并比较4组大鼠磨牙区段牙槽骨骨密度(bone mineral density,BMD);颌骨第一磨牙区段制取切片,进行HE染色观察各组牙槽骨组织形态;切片进行免疫组化染色比较各组根周牙槽骨中Runt相关转录因子2(Runt-related transcription factor 2,RUNX2)的表达.结果:BMD测定结果中,PTHrP组明显高于OVX组(P＜0.05),且与E2组及sham-OVX组间无明显差异(P＞0.05);HE染色显示,PTHrP组、E2组和sham-OVX组牙槽骨形态均较完整,而OVX组牙槽骨吸收较多;免疫组化染色显示,PTHrP组牙槽骨的RUNX2的表达明显高于于其余三组,差异有统计学意义(P＜0.05).结论:PTHrP可能对去卵巢大鼠牙槽骨的骨形成有促进作用.
목적:관찰갑상방선격소상관단백(parathyroid hormone-related protein,PTHrP)대거란소대서아조골적영향.방법:선용80지5월령건강자성대서,수궤선기중60지행쌍측란소적제술(ovariectomized,OVX),령외20지행가수술불거란소;6주후,60지거제란소적대서재수궤분위3조:PTHrP조주사PTHrP,자격소조(E2조)주사분갑산자이순,안위제조(OVX조)주사생리염수,매조20지;가수술적대서20지작위공백대조조(sham-OVX조)역주사생리염수.급약60 d후,처사대서해부분리우측하합골,측정병비교4조대서마아구단아조골골밀도(bone mineral density,BMD);합골제일마아구단제취절편,진행HE염색관찰각조아조골조직형태;절편진행면역조화염색비교각조근주아조골중Runt상관전록인자2(Runt-related transcription factor 2,RUNX2)적표체.결과:BMD측정결과중,PTHrP조명현고우OVX조(P＜0.05),차여E2조급sham-OVX조간무명현차이(P＞0.05);HE염색현시,PTHrP조、E2조화sham-OVX조아조골형태균교완정,이OVX조아조골흡수교다;면역조화염색현시,PTHrP조아조골적RUNX2적표체명현고우우기여삼조,차이유통계학의의(P＜0.05).결론:PTHrP가능대거란소대서아조골적골형성유촉진작용.