西部中医药
西部中醫藥
서부중의약
GANSU JOURNAL OF TRADITIONAL CHINESE MEDICINE
2014年
9期
24-26,27
,共4页
地补更年安颗粒%质量标准%薄层色谱法%五味子醇甲
地補更年安顆粒%質量標準%薄層色譜法%五味子醇甲
지보경년안과립%질량표준%박층색보법%오미자순갑
DiBu GengNianAn granules%quality standard%TLC%schisandrin
目的：建立地补更年安颗粒的质量控制标准。方法：采用薄层色谱法对地补更年安颗粒中五味子、葛根、当归进行定性鉴别，测定制剂中五味子醇甲的含量，色谱柱为Dikma Diamonsil-C18（200 mm×4.6 mm，5μm）；流动相为乙腈-水（45：55）；流速1.0 mL/min，检测波长250 nm，进样量20μL。结果：薄层鉴别的色谱斑点清晰，阴性对照无干扰；五味子醇甲含量在1.26～20.16μg范围内线性关系良好，平均回收率为99.4％， RSD=0.5％。结论：该方法操作简便、重复性好、专属性强、可用于地补更年安颗粒的质量控制。
目的：建立地補更年安顆粒的質量控製標準。方法：採用薄層色譜法對地補更年安顆粒中五味子、葛根、噹歸進行定性鑒彆，測定製劑中五味子醇甲的含量，色譜柱為Dikma Diamonsil-C18（200 mm×4.6 mm，5μm）；流動相為乙腈-水（45：55）；流速1.0 mL/min，檢測波長250 nm，進樣量20μL。結果：薄層鑒彆的色譜斑點清晰，陰性對照無榦擾；五味子醇甲含量在1.26～20.16μg範圍內線性關繫良好，平均迴收率為99.4％， RSD=0.5％。結論：該方法操作簡便、重複性好、專屬性彊、可用于地補更年安顆粒的質量控製。
목적：건입지보경년안과립적질량공제표준。방법：채용박층색보법대지보경년안과립중오미자、갈근、당귀진행정성감별，측정제제중오미자순갑적함량，색보주위Dikma Diamonsil-C18（200 mm×4.6 mm，5μm）；류동상위을정-수（45：55）；류속1.0 mL/min，검측파장250 nm，진양량20μL。결과：박층감별적색보반점청석，음성대조무간우；오미자순갑함량재1.26～20.16μg범위내선성관계량호，평균회수솔위99.4％， RSD=0.5％。결론：해방법조작간편、중복성호、전속성강、가용우지보경년안과립적질량공제。
Objective:To establish the standard of quality control for DiBu GengNianAn granules. Methods:WuWeiZi (Schisandra chinensis), GeGen (Lobed Kudzuvine Root) and DangGui (Angelica sinensis) of DiBu Geng-NianAn granules were identified qualitatively by thin-layer chromatography (TLC), the contents of schisandrin were measured in the preparation, chromatographic column: Dikma Diamonsil-C18 (200 mm ×4.6 mm, 5μm); mobile phase:acetonitrile-water (45:55);mobile phase:1.0 mL/min, detection wavelength:250nm, sample size:20μL. Re-sults:TCL spotting was clear, and the blank tests showed no interference;schisandrin represented good linear rela-tionship in the range between 1.26 and 20.16 μg, average recovery rate was 99.4%, RSD=0.5%. Conclusion:The method, simple, accurate and specific, could be used for quality control of DiBu GengNianAn granules.
