CAJ | 학술논문

目的：建立三黄中空栓中黄芩苷和盐酸小檗碱的含量测定方法。方法：采用高效液相色谱法，色谱柱为Waters Symmetry Shield-C18柱（4.6 mm×250 mm，5μm），以0.1%磷酸水溶液-乙腈（50：50）为流动相，流速为1.0 mL/min，检测波长265 nm，柱温为30℃进行检测。结果：黄芩苷线性范围为5.0～101.0μg/mL，平均加样回收率为98.80%（RSD%=1.17%)；盐酸小檗碱线性范围为5.7～114.0μg/mL，平均加样回收率为99.00%（RSD%=1.15%)。结论：该法快速、简便，结果准确、可靠，可用于三黄中空栓中黄芩苷和盐酸小檗碱含量的测定。
목적：건립삼황중공전중황금감화염산소벽감적함량측정방법。방법：채용고효액상색보법，색보주위Waters Symmetry Shield-C18주（4.6 mm×250 mm，5μm），이0.1%린산수용액-을정（50：50）위류동상，류속위1.0 mL/min，검측파장265 nm，주온위30℃진행검측。결과：황금감선성범위위5.0～101.0μg/mL，평균가양회수솔위98.80%（RSD%=1.17%)；염산소벽감선성범위위5.7～114.0μg/mL，평균가양회수솔위99.00%（RSD%=1.15%)。결론：해법쾌속、간편，결과준학、가고，가용우삼황중공전중황금감화염산소벽감함량적측정。
Objective: To establish content determination methods of baicalin and berberine hydrochloride contained in Sanhuang hollow suppository. Methods: HPLC was adopted, chromatographic column: Waters Symmetry Shield-C18 column (4.6 mm ×250 mm , 5μm ), 0.1%phosphoric acid solution-acetonitrile (50:50) were taken as the mobile phase, flow rate was 1.0 mL/min, detection wavelength was 265 nm, column temperature:30℃. Results:Baicalin showed better linear relationship between 5.0 and 101.0μg/mL, average recovery rate was 98.80%(RSD=1.17%);berberine hydrochloride showed better linear relationship between 5.7 and 114.0μg/mL, average re-covery rate was 99.00%(RSD=1.15%). Conclusion:The method, rapid, simple, accurate and reliable, could be used for content determination of baicalin and berberine hydrochloride contained in Sanhuang hollow suppository.