CAJ | 학술논문

以酿酒酵母 FX-12-135为初始菌株，以有机硒含量为筛选指标，筛选高产富硒酵母菌。首先采用紫外诱变方法处理菌株，然后利用氦氖激光辐照处理进行选育，得到一株高产突变株 HN-14-109，其摇瓶富硒能力比初始菌株提高29.8％，且遗传特性稳定。表明紫外诱变复合氦氖激光辐照是提高酵母富硒能力的有效方法。
이양주효모 FX-12-135위초시균주，이유궤서함량위사선지표，사선고산부서효모균。수선채용자외유변방법처리균주，연후이용양내격광복조처리진행선육，득도일주고산돌변주 HN-14-109，기요병부서능력비초시균주제고29.8％，차유전특성은정。표명자외유변복합양내격광복조시제고효모부서능력적유효방법。
Using the content of organic selenium as screening index,a high selenium-enriched yeast strain HN-14-109 was obtained from Saccharomyces cerevisiae FX-12-135.The primitive strain was treated firstly with UV mutation,and then followed by He-Ne laser irradiation.Its selenium-enriched productivity was in-creased by 29.8% than that of the primitive strain in shake flask.Genetics experiment results indicated that the hereditary character of this strain was stable,which demonstrated that UV mutation combined with He-Ne laser irradiation was an efficient method for screening selenium-enriched yeast strain.