CAJ | 학술논문

四苯基乙烯（TPE）衍生物 TPE-2SO3 Na＋是一种水溶性的聚集诱导发光分子。研究了 pH 值对 TPE-2SO3 Na＋荧光强度的影响、TPE-2SO3 Na＋对不同蛋白的荧光响应、MP/PNP对TPE-2SO3 Na＋荧光的猝灭效应以及 MP存在下TPE-2SO3 Na＋对不同类型蛋白的荧光响应。结果表明，TPE-2SO3 Na＋的荧光强度随着 pH 值的增大而减弱；在pH值8.0下，TPE-2SO3 Na＋对不同类型蛋白的荧光响应不同，MPH、BSA、Pap使TPE-2SO3 Na＋荧光强度增强，增强顺序为：BSA＞MPH＞Pap，且蛋白浓度越大，TPE-2SO3 Na＋荧光增强效果越明显；MP和 PNP都会对 TPE-2SO3 Na＋产生荧光猝灭，并且PNP的猝灭效率是MP的6倍。用MP猝灭TPE-2SO3 Na＋检测蛋白后发现BSA、Pap、Hb有荧光响应，通过加入 MP能够使TPE-2SO3 Na＋特异性识别BSA，为今后进一步的荧光探针筛选鉴别蛋白方法的建立奠定了基础。
사분기을희（TPE）연생물 TPE-2SO3 Na＋시일충수용성적취집유도발광분자。연구료 pH 치대 TPE-2SO3 Na＋형광강도적영향、TPE-2SO3 Na＋대불동단백적형광향응、MP/PNP대TPE-2SO3 Na＋형광적졸멸효응이급 MP존재하TPE-2SO3 Na＋대불동류형단백적형광향응。결과표명，TPE-2SO3 Na＋적형광강도수착 pH 치적증대이감약；재pH치8.0하，TPE-2SO3 Na＋대불동류형단백적형광향응불동，MPH、BSA、Pap사TPE-2SO3 Na＋형광강도증강，증강순서위：BSA＞MPH＞Pap，차단백농도월대，TPE-2SO3 Na＋형광증강효과월명현；MP화 PNP도회대 TPE-2SO3 Na＋산생형광졸멸，병차PNP적졸멸효솔시MP적6배。용MP졸멸TPE-2SO3 Na＋검측단백후발현BSA、Pap、Hb유형광향응，통과가입 MP능구사TPE-2SO3 Na＋특이성식별BSA，위금후진일보적형광탐침사선감별단백방법적건립전정료기출。
Tetraphenylethylene derivative TPE-2SO3 Na+ is one of the water soluble aggregation-induced e-mission(AIE)molecule.In this work,the effect of pH value on fluorescence intensity of TPE-2SO3 Na+,the quenching influence of MP and PNP on fluorescence intensity of TPE-2SO3 Na+,and the fluorescence response of TPE-2SO3 Na+ to different proteins in absence and presence of MP have been studied.Results show that,flu-orescence intensity of TPE-2SO3 Na+ decreases with the increasing of pH value;while at pH value of 8.0, MPH,BSA and Pap have higher fluorescence intensity to TPE-2SO3 Na+,the order is followed:BSA>MPH>Pap;fluorescence quenching of TPE-2SO3 Na+ by MP and PNP has been made,quenching efficiency of PNP is 6 times of MP;BSA can be specially identified by TPE-2SO3 Na+ in the presence of MP.This method lays a foun-dation for proteins identification by fluorescence probe.