CAJ | 학술논문

目的：探讨丹参对体外培养大鼠成纤维样滑膜细胞增殖及 bax、bcl-2表达的影响。方法取浓度为1.5×10-5个/ml的 RSC-364成纤维样滑膜细胞悬液100μl加入细胞培养板，培养24 h，加入含不同浓度丹参的培养液，采用 MTT法分析丹参对成纤维样滑膜细胞增殖抑制作用及其时效关系；免疫组化法检测 bcl-2、bax表达情况。结果丹参可明显抑制大鼠成纤维样滑膜细胞增殖，并显示出一定的量效和时效关系。丹参可显著上调大鼠成纤维样滑膜细胞 bax表达，下调 bcl-2表达，与空白对照组比较差异有统计学意义（ P <0.05）。结论丹参对体外培养大鼠成纤维样滑膜细胞的增殖具有抑制效应，其作用机制可能与调控 bax、bcl-2表达有关。
목적：탐토단삼대체외배양대서성섬유양활막세포증식급 bax、bcl-2표체적영향。방법취농도위1.5×10-5개/ml적 RSC-364성섬유양활막세포현액100μl가입세포배양판，배양24 h，가입함불동농도단삼적배양액，채용 MTT법분석단삼대성섬유양활막세포증식억제작용급기시효관계；면역조화법검측 bcl-2、bax표체정황。결과단삼가명현억제대서성섬유양활막세포증식，병현시출일정적량효화시효관계。단삼가현저상조대서성섬유양활막세포 bax표체，하조 bcl-2표체，여공백대조조비교차이유통계학의의（ P <0.05）。결론단삼대체외배양대서성섬유양활막세포적증식구유억제효응，기작용궤제가능여조공 bax、bcl-2표체유관。
Objective To explore the effect of Salvia Miltiorrhiza on fibroblastic synovial cells of bax/bcl-2 ex-pressions and proliferation culture in rats in vitro. Methods A concentration of 1. 5 × 10 -5/ ml RSC-364 fibroblastic synovial cells of 100 ul cell suspension was added with the cell culture plate,and was cultivated for 24 h,and then differ-ent concentrations of Salvia Miltiorrhiza culture medium were added. Inhibition effect of Salvia Miltiorrhiza on fibroblastic synovial cell proliferation and its time-effect relationship were analyzed using MTT method;bcl-2 and bax expressions were detected using immunohistochemical method. Results Salvia Miltiorrhiza was able to inhibit the cell proliferation of fibroblastic synovial cells in rats,and it showed a certain degree of concentration-response and time-effect relationships. Salvia Miltiorrhiza could significantly increase the bax expression of fibroblastic synovial cells,and reduce bcl-2 expres-sion of synovial cells,and the differences,compared with those in control group,were statistically significant( P <0. 05). Conclusion Salvia Miltiorrhiza can inhibit the proliferation of fibroblastic synovial cell culture in rats in vitro , and its mechanism of action may relate to regulation of bax and bcl-2 expressions.