CAJ | 학술논문

目的：观察补气活血方对慢性支气管炎肺气虚证大鼠肺组织 bcl-2及 bax表达的影响。方法 SD大鼠50只，随机选取10只作为空白对照组，其余40只采用改良烟熏法复制慢性支气管炎肺气虚证大鼠模型，造模成功后随机分为模型组、补气活血方高剂量组、补气活血方低剂量组、补肺汤组，每组10只。采用免疫组化法检测细胞凋亡相关基因蛋白 bcl-2、bax的表达情况。结果与空白对照组比较，模型组大鼠 bcl-2表达明显增加，bax表达降低（ P <0.01）；与模型组比较，补气活血方高剂量组、补气活血方低剂量组、补肺汤组 bcl-2蛋白表达降低，bax 蛋白表达增加（ P<0.01）；补气活血方高剂量组 bcl-2的表达较低剂量组和补肺汤组明显减少，bax的表达较低剂量组和补肺汤组明显增多（P<0.01）；补气活血方低剂量组 bcl-2表达低于补肺汤组，bax表达高于补肺汤组（P <0.05）。结论补气活血方能够通过抑制细胞凋亡相关基因蛋白 bcl-2的表达，上调蛋白 bax的表达，促进炎性细胞凋亡，可作为预防和治疗慢性支气管炎的有效方法。
목적：관찰보기활혈방대만성지기관염폐기허증대서폐조직 bcl-2급 bax표체적영향。방법 SD대서50지，수궤선취10지작위공백대조조，기여40지채용개량연훈법복제만성지기관염폐기허증대서모형，조모성공후수궤분위모형조、보기활혈방고제량조、보기활혈방저제량조、보폐탕조，매조10지。채용면역조화법검측세포조망상관기인단백 bcl-2、bax적표체정황。결과여공백대조조비교，모형조대서 bcl-2표체명현증가，bax표체강저（ P <0.01）；여모형조비교，보기활혈방고제량조、보기활혈방저제량조、보폐탕조 bcl-2단백표체강저，bax 단백표체증가（ P<0.01）；보기활혈방고제량조 bcl-2적표체교저제량조화보폐탕조명현감소，bax적표체교저제량조화보폐탕조명현증다（P<0.01）；보기활혈방저제량조 bcl-2표체저우보폐탕조，bax표체고우보폐탕조（P <0.05）。결론보기활혈방능구통과억제세포조망상관기인단백 bcl-2적표체，상조단백 bax적표체，촉진염성세포조망，가작위예방화치료만성지기관염적유효방법。
Objective To observe the effect of the Buqi huoxue decoction on expressions of bcl-2 and bax in chronic bronchitis( CB)rats with deficiency of lung-qi. Methods Ten rats were randomly selected from 50 SD rats as control group,and models of the deficiency of lung-qi of chronic bronchitis were established using the improved smoking-fumigated method in the other 40 rats. The successful model rats were randomly divided into model group,high-dose group of Buqi huoxue decoction,low-dose group of Buqi huoxue decoction and tonifying lung recipe group( n =10 for each group). Expressions of apoptosis-related gene bcl-2 and bax were detected using immunohistochemistry method. Re-sults Compared with those in control group,the bcl-2 expression in the model group was significantly increased,while the bax expression was significantly decreased(P<0. 01);compared with those in model group,the bcl-2 expressions were significantly reduced,while the bax expressions were significantly increased in high-dose,low-dose and tonifying lung recipe groups(P<0. 01);the bcl-2 expression in high dose group was significantly decreased,while the bax ex-pression in high dose group was significantly increased than those in low dose and tonifying lung recipe groups( P <0. 01);the bcl-2 expression was significantly lower,while the bax expression was significantly higher in low-dose group than that in tonifying lung recipe group(P<0. 05). Conclusion The Buqi huoxue decoction can inhibit the expression of apoptosis-related gene bcl-2,increase the expression of bax in inflammatory cells and promote inflammatory cell apop-tosis,which can be used as an effective method of prevention and treatment of chronic bronchitis.