湖南农业科学
湖南農業科學
호남농업과학
HUNAN AGRICULTURAL SCIENCES
2014年
16期
1-4,8
,共5页
甘薯%茎尖脱毒%组织培养%激素浓度配比%愈伤组织诱导率%成苗率%苗高
甘藷%莖尖脫毒%組織培養%激素濃度配比%愈傷組織誘導率%成苗率%苗高
감서%경첨탈독%조직배양%격소농도배비%유상조직유도솔%성묘솔%묘고
sw eetpotato%A m erica SL-9%virus-free by stem-apex tissue culture%horm one concentration ratio%callus induction rate%plantlet rate%plantletheight
为探索高淀粉甘薯品种美国SL-9茎尖脱毒组织培养的最适激素浓度配比，选择6-BA和α-NAA两种激素，通过不同浓度的配比，利用线性综合评价模型对愈伤组织诱导率L、出芽（成苗）率M和芽长（苗高）N三个培养效果指标进行综合评价。结果表明，最适于该品种茎尖脱毒组织培养的培养基配方为MS＋1.00 mg/L 6-BA＋0.50 mg/Lα-NAA；在此培养基上，美国SL-9愈伤组织诱导率L为83.3%，出芽（成苗）率M为100%，且成苗速度快，培养时间短，接种培养40 d苗高达11.4 mm，优于其它激素浓度配比。
為探索高澱粉甘藷品種美國SL-9莖尖脫毒組織培養的最適激素濃度配比，選擇6-BA和α-NAA兩種激素，通過不同濃度的配比，利用線性綜閤評價模型對愈傷組織誘導率L、齣芽（成苗）率M和芽長（苗高）N三箇培養效果指標進行綜閤評價。結果錶明，最適于該品種莖尖脫毒組織培養的培養基配方為MS＋1.00 mg/L 6-BA＋0.50 mg/Lα-NAA；在此培養基上，美國SL-9愈傷組織誘導率L為83.3%，齣芽（成苗）率M為100%，且成苗速度快，培養時間短，接種培養40 d苗高達11.4 mm，優于其它激素濃度配比。
위탐색고정분감서품충미국SL-9경첨탈독조직배양적최괄격소농도배비，선택6-BA화α-NAA량충격소，통과불동농도적배비，이용선성종합평개모형대유상조직유도솔L、출아（성묘）솔M화아장（묘고）N삼개배양효과지표진행종합평개。결과표명，최괄우해품충경첨탈독조직배양적배양기배방위MS＋1.00 mg/L 6-BA＋0.50 mg/Lα-NAA；재차배양기상，미국SL-9유상조직유도솔L위83.3%，출아（성묘）솔M위100%，차성묘속도쾌，배양시간단，접충배양40 d묘고체11.4 mm，우우기타격소농도배비。
In quest of the stem -apex tissue culture medium at the optim al horm one concentration ratio for a sweet potato variety America SL -9 with high starch content, this study used the linear com prehensive evaluation model in the assessm ent of three effect indexes for the culture such as callus induction rate, plantletrate and plantletheight.T he results indicate thatthe optim alhorm one concentration ratio for the variety could be MS+1.00mg/L 6-B A +0.50 mg/Lα-NAA , in which its callus induction rate (L)= 83.3% and plantlet rate (M)= 100.0% with rapid germ ination and shortculture tim e,i.e.,11.4 mm in plantletheight40 d after culturing; which was better than thatof the otherhorm one concentration ratios.
