CAJ | 학술논문

目的：探讨Wnt信号通路的β- catenin和拮抗因子SFRP1在甲状腺乳头状癌（PTC）中的表达及意义。方法采用免疫组化PV-8000法检测78例PTC组织和24例正常甲状腺组织中β- catenin和Wnt拮抗因子SFRP1蛋白的表达情况。结果 PTC组织中β- catenin阳性表达率明显高于正常甲状腺组织（P＜0.01），而Wnt拮抗因子SFRP1蛋白在PTC组织中阳性表达率明显低于正常甲状腺组织（P＜0.01）。β- catenin和Wnt拮抗因子SFRP1蛋白表达均与PTC临床分期、淋巴结转移及患者年龄有关（P＜0.05或0.01），而均与患者性别、肿瘤大小无关（均P＞0.05）。Spearman相关分析显示在PTC组织中两者之间表达呈负相关（P＜0.01）。结论β- catenin和SFRP1蛋白表达异常可能与PTC的发生、发展有关。两种蛋白联合检测对判断PTC的恶性程度和病情进展有重要价值。
목적：탐토Wnt신호통로적β- catenin화길항인자SFRP1재갑상선유두상암（PTC）중적표체급의의。방법채용면역조화PV-8000법검측78례PTC조직화24례정상갑상선조직중β- catenin화Wnt길항인자SFRP1단백적표체정황。결과 PTC조직중β- catenin양성표체솔명현고우정상갑상선조직（P＜0.01），이Wnt길항인자SFRP1단백재PTC조직중양성표체솔명현저우정상갑상선조직（P＜0.01）。β- catenin화Wnt길항인자SFRP1단백표체균여PTC림상분기、림파결전이급환자년령유관（P＜0.05혹0.01），이균여환자성별、종류대소무관（균P＞0.05）。Spearman상관분석현시재PTC조직중량자지간표체정부상관（P＜0.01）。결론β- catenin화SFRP1단백표체이상가능여PTC적발생、발전유관。량충단백연합검측대판단PTC적악성정도화병정진전유중요개치。
Objective To investigate the expression of β- catenin of Wnt signaling pathway and its antagonist factor SFRP1 in papil ary thyroid carcinoma (PTC) and its clinicopathological significance. Methods The expression ofβ- catenin and SFRP1 was detected by immunohistochemical PV- 8000 method in 78 cases of papil ary thyroid carcinoma and 24 samples of normal thyroid tissue. Results β- catenin expression in PTC was significantly higher and SFRP1 expression was lower than that in normal thyroid tissue (both P<0.01). The expression ofβ- catenin and SFRP1 in PTC was correlated with clinical stage and lymph node metastasis and age of the patients (P<0.05 or P<0.01),while not correlated with sex and tumor size. Spearman cor-relation analysis showed that there was a negative correlation betweenβ- catenin and SFRP1 expression in PTC(P<0.01). Con-clusion The abnormal expression ofβ- catenin and SFRP1 may be involved in the pathogenesis of PTC. The combined detection of two proteins would help to assess the malignant degree and progression of PTC.