实用临床医药杂志
實用臨床醫藥雜誌
실용림상의약잡지
JOURNAL OF JIANGSU CLINICAL MEDICINE
2014年
17期
70-72
,共3页
严凤英%李晓洁%吕群%张小建
嚴鳳英%李曉潔%呂群%張小建
엄봉영%리효길%려군%장소건
体外受精%优质囊胚培养%胚胎卵裂球数%碎片评级
體外受精%優質囊胚培養%胚胎卵裂毬數%碎片評級
체외수정%우질낭배배양%배태란렬구수%쇄편평급
position fertilization%high quality blastocyst culture%embryo cleavage count%debris rating
目的：观察体外受精（IVF）治疗周期中体外培养优质囊胚形成情况，并分析其与D2D3胚胎卵裂球数及碎片评级关系。方法选择本院2013年11月-2014年1月卵裂期第3天（D3）冷冻后剩余胚胎，将其体外培养至囊胚期。观察优质囊胚形成情况。同时探讨其D2D3胚胎卵裂球数及碎片评级。结果 D3≥10个细胞的优质囊胚形成率为38．9％，7～9个细胞43．1％，比较无显著差异（P＞0．05）；7～9个细胞的优质囊胚形成率为43．1％，显著高于4～6个细胞10．2％，比较有显著差异（P＜0．05）；D24个细胞的优质囊胚形成率为43．6％，显著高于5个细胞28．9％，比较有显著差异（P＜0．05），并显著高于2～3个细胞数12．3％，比较有显著差异（P＜0．05）；5个细胞的优质囊胚形成率为28．9％，6个细胞23．4％，比较无显著差异（P＞0．05）；Ⅰ、Ⅱ级D3胚胎碎片的优质囊胚形成率为36．4％，显著高于Ⅲ、Ⅳ级6．1％，比较有显著差异（P＜0．05），Ⅰ、Ⅱ级D2胚胎碎片的优质囊胚形成率为35．3％，显著高于Ⅲ、Ⅳ级4．7％，比较有显著差异（P＜0．05）。结论延长体外受精培养可有效筛选发育潜力的胚胎。D3胚胎卵裂球数越多，优质囊胚越多。D2胚胎4个细胞，囊胚发育能力较好。Ⅰ、Ⅱ级D2、D3胚胎碎片的优质囊胚形成率较高。优质囊胚形成与D2、D3胚胎卵裂球数及碎片评级密切相关。
目的：觀察體外受精（IVF）治療週期中體外培養優質囊胚形成情況，併分析其與D2D3胚胎卵裂毬數及碎片評級關繫。方法選擇本院2013年11月-2014年1月卵裂期第3天（D3）冷凍後剩餘胚胎，將其體外培養至囊胚期。觀察優質囊胚形成情況。同時探討其D2D3胚胎卵裂毬數及碎片評級。結果 D3≥10箇細胞的優質囊胚形成率為38．9％，7～9箇細胞43．1％，比較無顯著差異（P＞0．05）；7～9箇細胞的優質囊胚形成率為43．1％，顯著高于4～6箇細胞10．2％，比較有顯著差異（P＜0．05）；D24箇細胞的優質囊胚形成率為43．6％，顯著高于5箇細胞28．9％，比較有顯著差異（P＜0．05），併顯著高于2～3箇細胞數12．3％，比較有顯著差異（P＜0．05）；5箇細胞的優質囊胚形成率為28．9％，6箇細胞23．4％，比較無顯著差異（P＞0．05）；Ⅰ、Ⅱ級D3胚胎碎片的優質囊胚形成率為36．4％，顯著高于Ⅲ、Ⅳ級6．1％，比較有顯著差異（P＜0．05），Ⅰ、Ⅱ級D2胚胎碎片的優質囊胚形成率為35．3％，顯著高于Ⅲ、Ⅳ級4．7％，比較有顯著差異（P＜0．05）。結論延長體外受精培養可有效篩選髮育潛力的胚胎。D3胚胎卵裂毬數越多，優質囊胚越多。D2胚胎4箇細胞，囊胚髮育能力較好。Ⅰ、Ⅱ級D2、D3胚胎碎片的優質囊胚形成率較高。優質囊胚形成與D2、D3胚胎卵裂毬數及碎片評級密切相關。
목적：관찰체외수정（IVF）치료주기중체외배양우질낭배형성정황，병분석기여D2D3배태란렬구수급쇄편평급관계。방법선택본원2013년11월-2014년1월란렬기제3천（D3）냉동후잉여배태，장기체외배양지낭배기。관찰우질낭배형성정황。동시탐토기D2D3배태란렬구수급쇄편평급。결과 D3≥10개세포적우질낭배형성솔위38．9％，7～9개세포43．1％，비교무현저차이（P＞0．05）；7～9개세포적우질낭배형성솔위43．1％，현저고우4～6개세포10．2％，비교유현저차이（P＜0．05）；D24개세포적우질낭배형성솔위43．6％，현저고우5개세포28．9％，비교유현저차이（P＜0．05），병현저고우2～3개세포수12．3％，비교유현저차이（P＜0．05）；5개세포적우질낭배형성솔위28．9％，6개세포23．4％，비교무현저차이（P＞0．05）；Ⅰ、Ⅱ급D3배태쇄편적우질낭배형성솔위36．4％，현저고우Ⅲ、Ⅳ급6．1％，비교유현저차이（P＜0．05），Ⅰ、Ⅱ급D2배태쇄편적우질낭배형성솔위35．3％，현저고우Ⅲ、Ⅳ급4．7％，비교유현저차이（P＜0．05）。결론연장체외수정배양가유효사선발육잠력적배태。D3배태란렬구수월다，우질낭배월다。D2배태4개세포，낭배발육능력교호。Ⅰ、Ⅱ급D2、D3배태쇄편적우질낭배형성솔교고。우질낭배형성여D2、D3배태란렬구수급쇄편평급밀절상관。
Obj ective To observe the position fertilization (IVF)treatment cycle of in vitro cultivation quality blastocyst formation and analyze its relationship with pieces of D2D3 embryo cleav-age count and rating.Methods Cleavage stage 3 day (D3 )frozen surplus embryos were selected and cultured to blastocyst stage.Quality of blastocyst formation was observed,and at the same time their D2D3 embryo cleavage count and debris were discussed.Results The high quality D3 10 or more cells blastocyst formation rate was 38.9%,which was almost equal to 43.1%of 7 to 9 cells blastocyst formation rate (P>0 .05 ).7 to 9 cell quality blastocyst formation rate was 43 .1%, which was significantly higher than 10.2%of 4 to 6 cells (P<0.05).D2 4 cell quality blastocyst formation rate was 43.1%,which was significantly higher than 28.9%of 5 cells and 12.3%of 2 to 3 cells (P<0 .05 ).5 cells quality blastocyst formation rate was 28 .9%,which was almost equal to 23 .4%of 6 cells blastocyst formation rate (P>0 .05 ).Ⅰand classⅡD3 embryos pieces of high quality blastocyst formation rate was 36.4%,which was significantly higher than 6.1%of gradeⅢorⅣ(P<0 .05 ).Ⅰand class ⅡD2 embryos pieces of high quality blastocyst formation rate was 35.3%,which was significantly higher than 4.7% of grade Ⅲ or Ⅳ (P<0.05).Conclusion Extending IVF can effectively filter the embryo developmental potential.The more D3 embryo cleavage count ,the more high-quality blastocysts .D2 4 cells ,embryo blastocyst development ability is good.Ⅰand classⅡD2 and D3 embryos pieces of high quality blastocyst formation rate is higher.High quality blastocyst formation and D2 and D3 embryo cleavage count and debris are closely related to the ratings.
