CAJ | 학술논문

目的：筛选肝肾阴虚型晚期结直肠癌（CRC）患者使用六味地黄颗粒前后的基因表达谱差异，探索六味地黄颗粒药效的基因组学机制。方法选取晚期肝肾阴虚型CRC患者以六味地黄颗粒进行干预，21 d为1个疗程。干预期间患者不接受其他西医及中医药治疗。21 d后再次提取外周血，进行基因芯片检测，并与干预前芯片结果进行自身对照分析。结果与干预前相比，干预后基因芯片检测发现129个差异基因条，其中128个上调，1个下调。基因功能（GO）富集分析结果显示，干预前后共发现254个基因GO存在显著差异（P＜0．05或P＜0．01）。在生物过程中，凝血功能相关的基因占41．5％；在细胞组成中，45．5％的差异基因与细胞质膜有关；在分子功能方面，64．9％的差异基因与结合有关。结论晚期CRC肝肾阴虚型患者应用六味地黄颗粒干预前后存在差异表达基因，功能分析显示六味地黄颗粒可增强患者凝血功能，增加钙离子结合。
목적：사선간신음허형만기결직장암（CRC）환자사용륙미지황과립전후적기인표체보차이，탐색륙미지황과립약효적기인조학궤제。방법선취만기간신음허형CRC환자이륙미지황과립진행간예，21 d위1개료정。간예기간환자불접수기타서의급중의약치료。21 d후재차제취외주혈，진행기인심편검측，병여간예전심편결과진행자신대조분석。결과여간예전상비，간예후기인심편검측발현129개차이기인조，기중128개상조，1개하조。기인공능（GO）부집분석결과현시，간예전후공발현254개기인GO존재현저차이（P＜0．05혹P＜0．01）。재생물과정중，응혈공능상관적기인점41．5％；재세포조성중，45．5％적차이기인여세포질막유관；재분자공능방면，64．9％적차이기인여결합유관。결론만기CRC간신음허형환자응용륙미지황과립간예전후존재차이표체기인，공능분석현시륙미지황과립가증강환자응혈공능，증가개리자결합。
Objective The genomics mechanism of Liuwei Dihuang Granule efficacy was ex-plored by selecting the different gene expression profiles before and after the application of Liuwei Di-huang Granule for patients with advanced colorectal cancer (CRC)due to yin deficiency of liver and kidney.Methods Liuwei Dihuang Granule was given to patients with advanced CRC due to yin defi-ciency of liver and kidney,21 days as a course.During treatment,no western medicines or other Chi-nese medicines were administrated.21 days later,peripheral blood was collected again to detect the gene chips.Then the chips after intervention were compared with themselves before intervention.Re-sults Compared with intervention before,the chips after intervention had 129 differential gene frag-ments,in which 128 were up-regulated and 1 was down-regulated.Gene function enrichment anal-ysis showed that a total of 254 gene ontologies (GO)had significant differences before and after inter-ventions (P<0.05 orP<0.01).In the biological process,coagulation-related genes accounted for 41.5%.In cellular components,45.5%differential cells was associated with plasma membrane while in molecular function,64.9% was correlated with combination.Conclusion Patients with advanced CRC due to yin deficiency of liver and kidney have differential gene expressions before and after the in-terventions of Liuwei Dihuang Granule,and GO analysis indicates that Liuwei Dihuang Granule can strengthen the coagulation function and combination with calcium of CRC patients.