实用临床医药杂志
實用臨床醫藥雜誌
실용림상의약잡지
JOURNAL OF JIANGSU CLINICAL MEDICINE
2014年
17期
1-4
,共4页
尹立国%张中原%王金林%李晓亮%高建亮
尹立國%張中原%王金林%李曉亮%高建亮
윤입국%장중원%왕금림%리효량%고건량
颅脑创伤%氧自由基线粒体信号通路%凋亡%神经行为学
顱腦創傷%氧自由基線粒體信號通路%凋亡%神經行為學
로뇌창상%양자유기선립체신호통로%조망%신경행위학
traumatic brain inj ury%the signal transduction pathway of oxidative stress%cyt-c%apoptosis neuropraxiology
目的:探讨脑创伤后单唾液酸四己糖神经节苷脂对氧自由基线粒体信号通路的影响。方法采用改进的Mar-marou′s法建立大鼠中度闭合性弥漫性颅脑损伤模型。应用免疫组化、TUNEL法对大鼠伤后皮质细胞色素c、凋亡细胞数进行动态观察。硫代巴比妥酸法测量丙二醛。结果伤后给予Edaravone进行干预自由基水平明显减少,细胞色素c表达和神经元细胞凋亡数降低;依达拉奉与单唾液酸四己糖神经节苷脂联合干预效果更为明显。结论颅脑创伤后单唾液酸神经节苷脂具有脑保护作用,其可能的机制之一是调节氧自由基线粒体信号通路而发挥作用。
目的:探討腦創傷後單唾液痠四己糖神經節苷脂對氧自由基線粒體信號通路的影響。方法採用改進的Mar-marou′s法建立大鼠中度閉閤性瀰漫性顱腦損傷模型。應用免疫組化、TUNEL法對大鼠傷後皮質細胞色素c、凋亡細胞數進行動態觀察。硫代巴比妥痠法測量丙二醛。結果傷後給予Edaravone進行榦預自由基水平明顯減少,細胞色素c錶達和神經元細胞凋亡數降低;依達拉奉與單唾液痠四己糖神經節苷脂聯閤榦預效果更為明顯。結論顱腦創傷後單唾液痠神經節苷脂具有腦保護作用,其可能的機製之一是調節氧自由基線粒體信號通路而髮揮作用。
목적:탐토뇌창상후단타액산사기당신경절감지대양자유기선립체신호통로적영향。방법채용개진적Mar-marou′s법건립대서중도폐합성미만성로뇌손상모형。응용면역조화、TUNEL법대대서상후피질세포색소c、조망세포수진행동태관찰。류대파비타산법측량병이철。결과상후급여Edaravone진행간예자유기수평명현감소,세포색소c표체화신경원세포조망수강저;의체랍봉여단타액산사기당신경절감지연합간예효과경위명현。결론로뇌창상후단타액산신경절감지구유뇌보호작용,기가능적궤제지일시조절양자유기선립체신호통로이발휘작용。
Obj ective To explore the influence of monosialotetera-hexosyl ganglioside on the signal transduction pathway of oxidative stress in rats with traumatic brain inj ury.Methods The improved model of moderate closed brain inj ury was established in rats by using of the method of Marmarou.After traumatic brain injury free radical,cyt-c and countity of apoptosis neural cell in the cortex of rats were observed dynamically with the method of TAB,immunohistochemry and TUNEL.Results The treatment with edaravone after traumatic brain inj ury was able to decrease the level of free radical expression of cyt-c and countity of apoptosis nerve cell.Edaravone could im-prove the defective degree of neurophysiological functions at 72 hours after traumatic brain inj ury. Compared with edaravone,edaravone combined with ganglioside had a more apparent efficacy.Con-clusion Ganglioside has a cerebral protective effect,and one of the mechanisms is working by ad-justing the signal transduction pathway of oxidative stress.