中华临床医师杂志(电子版)
中華臨床醫師雜誌(電子版)
중화림상의사잡지(전자판)
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2014年
18期
3323-3327
,共5页
宋景春%林兆奋%王浈%杨洋%陈自力
宋景春%林兆奮%王湞%楊洋%陳自力
송경춘%림조강%왕정%양양%진자력
弥漫性血管内凝血%模型,动物%小鼠
瀰漫性血管內凝血%模型,動物%小鼠
미만성혈관내응혈%모형,동물%소서
Disseminated intravascular coagulation%Models,animal%Mice
目的:应用盲肠结扎穿孔术(CLP)建立小鼠弥漫性血管内凝血(DIC)动物模型。方法将昆明小鼠随机分为假手术组(sham)、中度 CLP 组(mCLP)和重度 CLP 组(hCLP),检测术后即刻、2、6、12、24、48、72 h共7个时间点的外周血血小板计数(PLT)、谷丙转氨酶(ALT)、肌酐(Cr)、凝血酶原时间(PT)、部分凝血活酶时间(APTT)、纤维蛋白原(FIB)、D-二聚体,计算72 h各组存活率,并进行组织病理切片观察。结果术后72 h小鼠sham组存活率均为100%, mCLP组存活率为60%,hCLP组存活率为10%(P<0.05)。与sham组相比,mCLP和hCLP组小鼠CLP术后6 h PLT、FIB明显下降,APTT+PT延长,D-二聚体、ALT升高,术后12 h Cr升高(P均<0.05),组织病理显示肺和肠系膜出现不同程度的微血栓形成。结论 CLP 小鼠模型能够体现类似临床的DIC的发展过程,重度CLP小鼠模型可作为脓毒症性DIC研究的动物模型。
目的:應用盲腸結扎穿孔術(CLP)建立小鼠瀰漫性血管內凝血(DIC)動物模型。方法將昆明小鼠隨機分為假手術組(sham)、中度 CLP 組(mCLP)和重度 CLP 組(hCLP),檢測術後即刻、2、6、12、24、48、72 h共7箇時間點的外週血血小闆計數(PLT)、穀丙轉氨酶(ALT)、肌酐(Cr)、凝血酶原時間(PT)、部分凝血活酶時間(APTT)、纖維蛋白原(FIB)、D-二聚體,計算72 h各組存活率,併進行組織病理切片觀察。結果術後72 h小鼠sham組存活率均為100%, mCLP組存活率為60%,hCLP組存活率為10%(P<0.05)。與sham組相比,mCLP和hCLP組小鼠CLP術後6 h PLT、FIB明顯下降,APTT+PT延長,D-二聚體、ALT升高,術後12 h Cr升高(P均<0.05),組織病理顯示肺和腸繫膜齣現不同程度的微血栓形成。結論 CLP 小鼠模型能夠體現類似臨床的DIC的髮展過程,重度CLP小鼠模型可作為膿毒癥性DIC研究的動物模型。
목적:응용맹장결찰천공술(CLP)건립소서미만성혈관내응혈(DIC)동물모형。방법장곤명소서수궤분위가수술조(sham)、중도 CLP 조(mCLP)화중도 CLP 조(hCLP),검측술후즉각、2、6、12、24、48、72 h공7개시간점적외주혈혈소판계수(PLT)、곡병전안매(ALT)、기항(Cr)、응혈매원시간(PT)、부분응혈활매시간(APTT)、섬유단백원(FIB)、D-이취체,계산72 h각조존활솔,병진행조직병리절편관찰。결과술후72 h소서sham조존활솔균위100%, mCLP조존활솔위60%,hCLP조존활솔위10%(P<0.05)。여sham조상비,mCLP화hCLP조소서CLP술후6 h PLT、FIB명현하강,APTT+PT연장,D-이취체、ALT승고,술후12 h Cr승고(P균<0.05),조직병리현시폐화장계막출현불동정도적미혈전형성。결론 CLP 소서모형능구체현유사림상적DIC적발전과정,중도CLP소서모형가작위농독증성DIC연구적동물모형。
Objective To construct disseminated intravascular coagulation(DIC) model in mice with cecal ligation and puncture. Methods KM mice were randomized into sham-operated group, middle grade group for cecal ligation and puncture(mCLP group) and high grade group for CLP(hCLP group). Blood were detected in immediately, 2 hours, 6 hours, 12 hours, 24 hours, 48 hours and 72 hours after CLP for platelet count, prothrombin time(PT), activated partial thromboplastin time(APTT), fibrinogen(FIB), D-dimer, alanine aminotransferase(ALT) and creatinine(Cr). Survival analysis at 72 h after CLP for each group was calculated, and pathological changes for mesenterium and lung were also observed at every time point after CLP. Results The survival rates of sham group, mCLP group and hCLP group were 100%, 60%and 10%, respectively. Compared with the sham group, the platelet count and FIB of the mCLP group and hCLP group decreased markedly, PT and APTT prolonged, D-dimer and ALT increased at 6 hours after CLP. Tissue edema and inflammatory cell infiltration were typical pathological changes in the tissues of mesenterium and lung in CLP mice. Conclusion CLP model in mice show similar pathophysiological with DIC and is fit to be a reliable model to study DIC.