中华临床医师杂志(电子版)
中華臨床醫師雜誌(電子版)
중화림상의사잡지(전자판)
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2014年
18期
3303-3306
,共4页
佟广辉%张晓平%佟威威%刘勇
佟廣輝%張曉平%佟威威%劉勇
동엄휘%장효평%동위위%류용
离心法%凝血酶原时间%凝血酶时间%纤维蛋白原%活化部分凝血活酶时间
離心法%凝血酶原時間%凝血酶時間%纖維蛋白原%活化部分凝血活酶時間
리심법%응혈매원시간%응혈매시간%섬유단백원%활화부분응혈활매시간
Centrifugation%Prothrombin time%Thrombin time%Fibrinogen%Activated partial thromboplastin time
目的:探讨不同离心速度和时间对不同人群凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)和纤维蛋白原(FIB)检测结果的影响。方法收集新生儿和婴儿新鲜血液标本共60例,另收集儿童及成人标本各60例,每例标本平均分成三份,分别以2500 r/min离心15 min,3500 r/min离心5 min和3000 r/min 离心10 min(对照组)。利用ACL-TOP全自动血凝分析仪及配套试剂测定血浆的PT、APTT、TT和FIB,同时应用Coulter LH 750全自动血细胞分析仪测定血小板数量。结果不同离心条件对成人凝血结果没有影响(P>0.05);2500 r/min离心15 min和3500 r/min离心5 min与对照组3000 r/min离心10 min相比较,新生儿和婴儿 PT、APTT、TT和FIB均缩短(P<0.05);儿童只有PT和APTT缩短(P<0.05),TT和FIB与对照组的差异无统计学意义(P>0.05)。结论乏血小板血浆的制备对于凝血常规检测结果的准确性及可靠性有重要影响,应建立适宜不同人群的乏血小板血浆制备的最佳离心条件。
目的:探討不同離心速度和時間對不同人群凝血酶原時間(PT)、活化部分凝血活酶時間(APTT)、凝血酶時間(TT)和纖維蛋白原(FIB)檢測結果的影響。方法收集新生兒和嬰兒新鮮血液標本共60例,另收集兒童及成人標本各60例,每例標本平均分成三份,分彆以2500 r/min離心15 min,3500 r/min離心5 min和3000 r/min 離心10 min(對照組)。利用ACL-TOP全自動血凝分析儀及配套試劑測定血漿的PT、APTT、TT和FIB,同時應用Coulter LH 750全自動血細胞分析儀測定血小闆數量。結果不同離心條件對成人凝血結果沒有影響(P>0.05);2500 r/min離心15 min和3500 r/min離心5 min與對照組3000 r/min離心10 min相比較,新生兒和嬰兒 PT、APTT、TT和FIB均縮短(P<0.05);兒童隻有PT和APTT縮短(P<0.05),TT和FIB與對照組的差異無統計學意義(P>0.05)。結論乏血小闆血漿的製備對于凝血常規檢測結果的準確性及可靠性有重要影響,應建立適宜不同人群的乏血小闆血漿製備的最佳離心條件。
목적:탐토불동리심속도화시간대불동인군응혈매원시간(PT)、활화부분응혈활매시간(APTT)、응혈매시간(TT)화섬유단백원(FIB)검측결과적영향。방법수집신생인화영인신선혈액표본공60례,령수집인동급성인표본각60례,매례표본평균분성삼빈,분별이2500 r/min리심15 min,3500 r/min리심5 min화3000 r/min 리심10 min(대조조)。이용ACL-TOP전자동혈응분석의급배투시제측정혈장적PT、APTT、TT화FIB,동시응용Coulter LH 750전자동혈세포분석의측정혈소판수량。결과불동리심조건대성인응혈결과몰유영향(P>0.05);2500 r/min리심15 min화3500 r/min리심5 min여대조조3000 r/min리심10 min상비교,신생인화영인 PT、APTT、TT화FIB균축단(P<0.05);인동지유PT화APTT축단(P<0.05),TT화FIB여대조조적차이무통계학의의(P>0.05)。결론핍혈소판혈장적제비대우응혈상규검측결과적준학성급가고성유중요영향,응건립괄의불동인군적핍혈소판혈장제비적최가리심조건。
Objective To investigate effects of different centrifugation time and speed on prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB) in different people. Methods 60 cases of newborns and infants, 60 cases of children and 60 cases of adults fresh blood specimens were divided into three groups of each case, which were respectively centrifuged with 2 500 r/min speed, 15 min, 3 500 r/min speed, 5 min and 3 000 r/min speed, 10 min (control). The PT, APTT, TT and FIB results were tested by ACL-TOP full-automatic blood coagulation analyzer. The platelet of the blood plasma were measured by Coulter LH 750 full-automatic blood cell analyzer. Results There was no effect on the result of the adults blood clotting under different centrifugal conditions. PT, APTT, TT and FIB in the group of 2 500 r/min speed, 15 min and 3 500 r/min speed, 5 min were lower than those of control group in newborns and infants(P<0.05).PT, APTT were lower than those of control group in children(P<0.05), but there was no significant difference between TT and FIB. Conclusion Best centrifugal condition of spent platelet plasma should be established for different people.