CAJ | 학술논문

目的：了解近年来山东地区汉族丙型肝炎病毒（HCV）感染者基因型的分布特点及与 HCV-RNA病毒载量的相关性。方法抽取山东省立医院门诊及住院汉族丙型肝炎患者实时荧光定量聚合酶链反应检测RNA阳性标本153例，用焦磷酸测序法进行基因分型。结果共检出5种基因亚型，1b、2a、1a、3a、3b比例依次为65．36％、28．76％、3．92％、1．32％、0．65％。1b亚型病毒载量高于2a ，其他亚型病毒载量之间比较，差异无统计学意义（P＞0．05）。结论山东地区汉族HCV基因型以1b为主，其次为2a ，且1b亚型病毒载量高于2a。
목적：료해근년래산동지구한족병형간염병독（HCV）감염자기인형적분포특점급여 HCV-RNA병독재량적상관성。방법추취산동성립의원문진급주원한족병형간염환자실시형광정량취합매련반응검측RNA양성표본153례，용초린산측서법진행기인분형。결과공검출5충기인아형，1b、2a、1a、3a、3b비례의차위65．36％、28．76％、3．92％、1．32％、0．65％。1b아형병독재량고우2a ，기타아형병독재량지간비교，차이무통계학의의（P＞0．05）。결론산동지구한족HCV기인형이1b위주，기차위2a ，차1b아형병독재량고우2a。
Objective To study the latest distribution of hepatitis C genotypes and the correlation between hepatitis C genotypes and viral loads among chronic hepatitis C patients of Han nationality in Shandong region .Meth-ods One hundred and fifty three HCV RNA positive cases from inpatients and outpatients of Han nationality in Shandong provincial hospital were detected by real-time fluorescent quantitative PCR (FQ-PCR) ,followed by pyro-phosphate sequencing method for genotyping .Results Five subtypes including HCV 1b ,2a ,1a ,3a ,3b were detected at frequencies of 65 .36% ,28 .76% ,3 .92% ,1 .31% ,0 .65% .HCV1b was associated with a significantly higher viral load compared to HCV2a(P< 0 .05) .There was no significant differences among the other subtypes .Conclusion HCV 1b is the most predominant subtypes among hepatitis C patients of Han nationality in Shandong ,followed by 2a ,and the viral load of HCV 1b was higher than 2a .