CAJ | 학술논문

目的：研究CD117、Nestin、CD133和Ki 67在卵巢上皮性癌（ EOC）中的表达，探讨其表达与卵巢癌临床病理参数及预后的相关性。方法选择河北省邯郸市中心医院病理存档的石蜡标本80例，其中20例正常卵巢和60例卵巢上皮癌，采用免疫组化法（ SP法）检测CD117、Nestin、CD133和Ki67的表达情况。结果 CD117、Nestin、CD 133和Ki67在卵巢上皮性癌中的阳性表达率明显高于在正常卵巢组织（ P ＜0?．05）。 CD117、Nestin及CD133在EOC组中的表达水平与其FIGO分期及病理分级有关，在Ⅲ～Ⅳ期、高级别组（G3）比Ⅰ～Ⅱ期、低级别组（G1、G2）表达率高（ P＜0．05）。 Ki67表达与卵巢癌组织分化程度、CA125水平相关差异有统计学意义（ P ＜0．05）。通过Kaplan-Meier生存曲线了解CD 117、Nestin、CD133在预测卵巢癌预后中的价值。结论 CD117、Nestin、CD133和Ki67的阳性表达在卵巢上皮癌的发生、发展中发挥重要，CD117、Nestin、CD133可能作为卵巢癌干细胞标志物用于判断卵巢上皮性癌的预后。
목적：연구CD117、Nestin、CD133화Ki 67재란소상피성암（ EOC）중적표체，탐토기표체여란소암림상병리삼수급예후적상관성。방법선택하북성함단시중심의원병리존당적석사표본80례，기중20례정상란소화60례란소상피암，채용면역조화법（ SP법）검측CD117、Nestin、CD133화Ki67적표체정황。결과 CD117、Nestin、CD 133화Ki67재란소상피성암중적양성표체솔명현고우재정상란소조직（ P ＜0?．05）。 CD117、Nestin급CD133재EOC조중적표체수평여기FIGO분기급병리분급유관，재Ⅲ～Ⅳ기、고급별조（G3）비Ⅰ～Ⅱ기、저급별조（G1、G2）표체솔고（ P＜0．05）。 Ki67표체여란소암조직분화정도、CA125수평상관차이유통계학의의（ P ＜0．05）。통과Kaplan-Meier생존곡선료해CD 117、Nestin、CD133재예측란소암예후중적개치。결론 CD117、Nestin、CD133화Ki67적양성표체재란소상피암적발생、발전중발휘중요，CD117、Nestin、CD133가능작위란소암간세포표지물용우판단란소상피성암적예후。
O bjective To investigate the expressions of CD117,Nestin,CD133 and Ki67 in epithelial ovarian cancer (EOC),and to explore the correlation between the expressions and clinicopathologic parameters as well as patient ’s prognosis. Metho ds Eighty paraffin specimens kept in Handan Central Hospital were enrolled in the study ,in which,there were 20 cases of normal ovarian tissues and 60 cases of EOC.The expressions of CD117,Nestin,CD133 and Ki67 were detected by immunohistochemistry ( SP).Results The positive expression rates of CD117, Nestin, CD133 and Ki67in EOC were significantly higher than those in normal ovarian tissues ( P <0.05).The expression levels of CD117,Nestin and CD133 in EOC were correlated to FIGO staging and pathological classification ,which in stageⅢor stageⅣ,G3 were significantly higher than those in stageⅠor stageⅡ,G1 or G2 ( P <0.05).The expression of Ki67 was closely related with differentiation degree of EOC tissues and the expression levels of CA 125 ( P <0.05).The value of detecting CD117,Nestin,CD133 in evaluating the prognosis of patients with EOC could be understand by means of Kaplan-Meier survival curve .Conclusion The positive expressions of CD117, Nestin, CD133 and Ki67 play an important role in the pathogenesis and development of EOC , and CD117,Nestin,CD133 can be used as EOC stem cell markers to evaluate the prognosis of patients with EOC .