CAJ | 학술논문

以1对甘蓝型黄籽（L2）、黑籽（L1）油菜的近等基因系为材料,研究了木质素生物合成的部分关键酶4CL、F5H和CAD在黄、黑籽油菜的不同组织中的表达差异。结果表明：4CL、F5H和CAD在甘蓝型黄、黑籽油菜不同器官和组织中的表达存在差异,特别是在种子中。其中,在同一组织器官中,4CL的不同种皮颜色之间的甘蓝型油菜中的表达差异明显;F5H在甘蓝型黄、黑籽油菜中的花与在30 d种子中的表达差异明显;CAD在种子的不同发育期中的表达差异明显。因此,这几种酶在甘蓝型黄、黑籽油菜种子中的表达差异,特别是在甘蓝型黄籽油菜的30 d种子中的表达都明显低于相应的黑籽油菜,说明这些酶的转录水平是导致木质素在甘蓝型黄、黑籽油菜之间存在表达差异的主要原因之一,也可能是形成甘蓝型黄、黑籽油菜皮壳差异的重要原因之一。
이1대감람형황자（L2）、흑자（L1）유채적근등기인계위재료,연구료목질소생물합성적부분관건매4CL、F5H화CAD재황、흑자유채적불동조직중적표체차이。결과표명：4CL、F5H화CAD재감람형황、흑자유채불동기관화조직중적표체존재차이,특별시재충자중。기중,재동일조직기관중,4CL적불동충피안색지간적감람형유채중적표체차이명현;F5H재감람형황、흑자유채중적화여재30 d충자중적표체차이명현;CAD재충자적불동발육기중적표체차이명현。인차,저궤충매재감람형황、흑자유채충자중적표체차이,특별시재감람형황자유채적30 d충자중적표체도명현저우상응적흑자유채,설명저사매적전록수평시도치목질소재감람형황、흑자유채지간존재표체차이적주요원인지일,야가능시형성감람형황、흑자유채피각차이적중요원인지일。
The differential expression of 4CL, F5H and CAD, which were the lignin thetic enzymes were investigated in different organizations, with 1 pair nearisogenic seed （L2）, black seed （L1） rapes. The results show that the expression of4CL, F5H related biosyn- lines of yellow and CAD werediversified in different organs and tissues, especially in the seeds, of black or yellow seed rapes. That is, the expression of 4CL in the same organs was different between the seedcoat colors of Brassica na- pus L.. The expression of F5 H was apparently different in the penetrance of yellow and black seeds in the flowers, in 30 days of seed of the rapes. The expression of CAD was apparently different in differ- ent development stages seeds of yellow and black Brassica napus L.. Therefore, the expressions of these enzymes were different between black- or yellow-seeded Brassica napus L. , especially, it was significantly lower in 30 days seed in the yellow-seeded Brassica napus L. than black-seeded Brassica napus L. , which suggested that the differences of these enzymes transcription should be one of the main reasons that cause the lignin differences of the yellow or black seeds in rapes, which may be one of the important reasons that led to husk difference of the black or yellow rapes.