CAJ | 학술논문

目的研究碳青霉烯类耐药鲍曼不动杆菌的耐药性及碳青霉烯酶基因的携带情况.方法连续收集2011年1月至2012年4月浙江省台州医院临床分离的碳青霉烯类耐药鲍曼不动杆菌75株.应用Vitek 2 Compact微生物鉴定仪进行细菌鉴定和药敏分析,并通过Hodge试验筛选产碳青霉烯酶的菌株,PCR扩增分析碳青霉烯酶的基因型.结果 75株碳青霉烯类耐药鲍曼不动杆菌均为多重耐药菌株,只对阿米卡星保持较高的敏感性,耐药率仅为13.3％ (10/75).其中,69株(92.0％)检测出OXA-23基因,67株(89.3％)检出OXA-51基因,未检出IMP、VIM和SIM酶基因.结论产OXA酶是本组碳青霉烯类耐药鲍曼不动杆菌的主要耐药机制,其中OXA-23和OXA-51是主要基因型.
목적 연구탄청매희류내약포만불동간균적내약성급탄청매희매기인적휴대정황.방법 련속수집2011년1월지2012년4월절강성태주의원림상분리적탄청매희류내약포만불동간균75주.응용Vitek 2 Compact미생물감정의진행세균감정화약민분석,병통과Hodge시험사선산탄청매희매적균주,PCR확증분석탄청매희매적기인형.결과 75주탄청매희류내약포만불동간균균위다중내약균주,지대아미잡성보지교고적민감성,내약솔부위13.3％ (10/75).기중,69주(92.0％)검측출OXA-23기인,67주(89.3％)검출OXA-51기인,미검출IMP、VIM화SIM매기인.결론 산OXA매시본조탄청매희류내약포만불동간균적주요내약궤제,기중OXA-23화OXA-51시주요기인형.
Objective To investigate drug resistance and carbapenemase genotypes of carbapenemresistant Acinetobacter baumannii.Methods A total of 75 clinical isolated strains of carbapenem-resistant Acinetobacter baumannii were collected from Taizhou Hospital in Zhejiang province during January 2011 and June 2012.Vitek 2 Compact microbial identification system was used for bacterial identification and drug susceptibility test,and modified Hodge test was used to screen strains producing carbapenemases.Genotypes of carbapenamases were determined by polymerase chain reaction.Results All 75 carbapenem-resistant Acinetobacter baumannii strains were resistant to most antibacterial agents except amikacin.The resistant rate to amikacin was 13.3％ (10/75).OXA-23 gene was positive in 69 strains (92.0％),and OXA-51 gene was positive in 67 strains (89.3％).No IMP,VIM and SIM gene was observed.Conclusion OXA enzyme is the main cause of drug resistance in this group of carbapenem-resistant Acinetobacter baumannii,and OXA-23 and OXA-51 genes are the most popular carbapenemnases coding genes.