中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
10期
2272-2274
,共3页
骨形态发生蛋白%人工骨替代材料%骨缺损
骨形態髮生蛋白%人工骨替代材料%骨缺損
골형태발생단백%인공골체대재료%골결손
Bone morphogenetic protein%Artificial bone substitute materials%Bone defect
目的 观察重组人骨形态发生蛋白-2(rhBMP-2)复合β-磷酸三钙(β-TCP)及无机牛骨(Bio-Oss)对骨缺损修复能力的影响.方法 拔除10条Beagle犬下颌前磨牙,3个月骨质愈合后预备种植窝并制备5 mm×4 mm×3 mm骨缺损区,植入种植体,并分别在骨缺损区填入β-TCP、rhBMP-2/β-TCP复合物、Bio-Oss或rhBMP-2/Bio-Oss复合物;空白对照组不充填.术后4、12周处死犬,行大体观察、X线观察和组织形态学分析,应用图像分析软件分析缺损区骨密度改变及新生骨生成率.结果 植入骨替代材料的各实验组均有较好的新生骨.4周后,β-TCP/BMP及Bio-Oss/BMP组缺损区骨密度分别为(36.70±1.73)％和(38.50±1.83)％,高于原材料组[(30.50±1.81)％和(31.48±1.86)％.12周后,β-TCP/BMP及Bio-Oss/BMP组骨密度分别为(52.84±1.85)％和(54.85±1.87)％,也高于原材料组[(43.65±1.81)％和(44.94±1.88)％],且复合rhBMP-2的实验组新骨生成率更高.结论 rhBMP-2与人工骨替代材料复合后能够有效促进种植体周骨缺损的修复.
目的 觀察重組人骨形態髮生蛋白-2(rhBMP-2)複閤β-燐痠三鈣(β-TCP)及無機牛骨(Bio-Oss)對骨缺損脩複能力的影響.方法 拔除10條Beagle犬下頜前磨牙,3箇月骨質愈閤後預備種植窩併製備5 mm×4 mm×3 mm骨缺損區,植入種植體,併分彆在骨缺損區填入β-TCP、rhBMP-2/β-TCP複閤物、Bio-Oss或rhBMP-2/Bio-Oss複閤物;空白對照組不充填.術後4、12週處死犬,行大體觀察、X線觀察和組織形態學分析,應用圖像分析軟件分析缺損區骨密度改變及新生骨生成率.結果 植入骨替代材料的各實驗組均有較好的新生骨.4週後,β-TCP/BMP及Bio-Oss/BMP組缺損區骨密度分彆為(36.70±1.73)％和(38.50±1.83)％,高于原材料組[(30.50±1.81)％和(31.48±1.86)％.12週後,β-TCP/BMP及Bio-Oss/BMP組骨密度分彆為(52.84±1.85)％和(54.85±1.87)％,也高于原材料組[(43.65±1.81)％和(44.94±1.88)％],且複閤rhBMP-2的實驗組新骨生成率更高.結論 rhBMP-2與人工骨替代材料複閤後能夠有效促進種植體週骨缺損的脩複.
목적 관찰중조인골형태발생단백-2(rhBMP-2)복합β-린산삼개(β-TCP)급무궤우골(Bio-Oss)대골결손수복능력적영향.방법 발제10조Beagle견하합전마아,3개월골질유합후예비충식와병제비5 mm×4 mm×3 mm골결손구,식입충식체,병분별재골결손구전입β-TCP、rhBMP-2/β-TCP복합물、Bio-Oss혹rhBMP-2/Bio-Oss복합물;공백대조조불충전.술후4、12주처사견,행대체관찰、X선관찰화조직형태학분석,응용도상분석연건분석결손구골밀도개변급신생골생성솔.결과 식입골체대재료적각실험조균유교호적신생골.4주후,β-TCP/BMP급Bio-Oss/BMP조결손구골밀도분별위(36.70±1.73)％화(38.50±1.83)％,고우원재료조[(30.50±1.81)％화(31.48±1.86)％.12주후,β-TCP/BMP급Bio-Oss/BMP조골밀도분별위(52.84±1.85)％화(54.85±1.87)％,야고우원재료조[(43.65±1.81)％화(44.94±1.88)％],차복합rhBMP-2적실험조신골생성솔경고.결론 rhBMP-2여인공골체대재료복합후능구유효촉진충식체주골결손적수복.
Objective To evaluate the bone repair capacity of β-tricalciumphosphate (β-TCP) or Bio-Oss combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) in Beagle dogs.Methods Ten healthy adult Beagle dogs were used in this study.The mandibular premolars of the dogs were extracted firstly.Three months later,dental implants were placed in the mandibular,and a 5 mm× 4 mm× 3 mm bone defect area was prepared on the buccal side of each implant.The bone defect areas were filled with β-TCP,rhBMP-2/β-TCP compound,Bio-Oss or rhBMP-2/Bio-Oss compound respectively.The bone defect areas were filled nothing as the blank.The dogs were sacrificed after 4 or 12 weeks.Samples were prepared for the gross observation,X-ray analysis and histomorphology analysis.Results The new bone formation was observed in all experiment groups.After 4 weeks,the bone density of materials combined with rhBMP-2 groups [(36.70 ± 1.73) ％ and (38.50 ± 1.83) ％] were higher than the raw materials groups [(30.50 ± 1.81)％ and (31.48 ± 1.86)％].After 12 weeks,the bone density of materials combined with rhBMP-2 groups [(52.84 ± 1.85) ％ and (54.85 ± 1.87) ％] were also higher than the raw materials groups [(43.65 ± 1.81) ％ and (44.94 ± 1.88) ％].And new bone formation rate of the materials combined with rhBMP-2 groups were higher than the raw materials groups at each evaluation time.Conclusion rhBMP-2 can promote the bone regeneration activity of the artificial bone substitute materials effectively.
