CAJ | 학술논문

目的观察丹参注射液关节腔内注射对膝骨性关节炎早期诱导型一氧化氮合酶(iNOS)的影响.方法将24只雄性大耳白兔随机分为A组(模型组)、B组(阳性对照组),C组(实验组),均用手术方法切开兔膝关节造成膝骨性关节炎(KOA)模型,后行石膏固定术；进而B组腔内注射玻璃酸钠0.3 ml/只、C组腔内注射丹参注射液0.3 ml/只、A组给予同体积的生理盐水,每周1次,延续4周.4周后处死兔,剥取滑膜、关节软骨检测iNOS含量.结果 B组滑膜组织及软骨中iNOS含量分别为(0.83±0.12) μmol/L和(0.13 ±0.06) μmol/L,与A组比较差异有统计学意义(P＜0.05)；C组滑膜组织及软骨中iNOS含量分别为(0.93±0.15) μmol/L和(0.20±0.08) μmol/L,与A组比较差异有统计学意义(P＜0.05)；但B组滑膜组织及软骨中iNOS含量与C组比较差异无统计学意义(P＞0.05).结论丹参注射液腔内注射可降低iNOS含量,抑制滑膜炎症,从而促进关节软骨的修复.
목적 관찰단삼주사액관절강내주사대슬골성관절염조기유도형일양화담합매(iNOS)적영향.방법 장24지웅성대이백토수궤분위A조(모형조)、B조(양성대조조),C조(실험조),균용수술방법절개토슬관절조성슬골성관절염(KOA)모형,후행석고고정술；진이B조강내주사파리산납0.3 ml/지、C조강내주사단삼주사액0.3 ml/지、A조급여동체적적생리염수,매주1차,연속4주.4주후처사토,박취활막、관절연골검측iNOS함량.결과 B조활막조직급연골중iNOS함량분별위(0.83±0.12) μmol/L화(0.13 ±0.06) μmol/L,여A조비교차이유통계학의의(P＜0.05)；C조활막조직급연골중iNOS함량분별위(0.93±0.15) μmol/L화(0.20±0.08) μmol/L,여A조비교차이유통계학의의(P＜0.05)；단B조활막조직급연골중iNOS함량여C조비교차이무통계학의의(P＞0.05).결론 단삼주사액강내주사가강저iNOS함량,억제활막염증,종이촉진관절연골적수복.
Objective To observe the effect of Intra-articular Injection of Salvia on knee osteoarthritis early by inducible nitric oxide synthase (iNOS),and to explore its therapeutic mechanism.Methods Twenty-four Japanese male white rabbits were made into knee osteoarthritis model and randomly divided into 3 groups:Group A (model group),B Group (Sham group) and Group C (experimental group).The rabbits of Group A,B,C were injected normal saline 0.3 ml,sodium hyaluronate 0.3ml and Salvia 0.3 ml,respectively.Each rabbits received injection 4 times.Four weeks after the administration,the levels of iNOS in synovial and articular cartilage were measured.Results There were significant differences between the levels of iNOS in synovial and articular cartilage of rabbits in Group A and B,Group A and C (P ＜ 0.05),and no significant differences between that of rabbits in Group B and C.Conclusion Intra-articular Injection of Salvia could inhibit the expression of iNOS to decrease its level in knee OA,and contribute to slow down the degeneration of articular cartilage.