CAJ | 학술논문

目的制备新型的包芯结构骨支架,比较其与聚乳酸聚羟基乙酸聚合物(PLGA)/β-磷酸三钙(TCP)复合支架的体外生物相容性.方法支架通过快速成型方法制作.亲水性通过吸水率评价；细胞在支架上的增殖能力通过噻唑蓝(MTT)比色法测定,成骨能力通过碱性磷酸酶活性(ALP)检测评价,生长情况通过扫描电镜观测.结果包芯结构组吸水率为(18.1±0.4)％,较对照组(2.6±0.3)％显著提高(P＜0.01),细胞和支架共培养6、10、14d,包芯结构组细胞活度吸光度(A)值分别为:0.28 ±0.09、0.60 ±0.10、0.76 ±0.10,ALP分别为:124.33±14.22、189.00±19.52、230.00±12.12,对照组A值为:0.19 ±0.09、0.36±0.05、0.46 ±0.12,ALP为:78.33±8.02、105.67±7.77、123.67±20.84;组间差异有统计学意义(P＜0.05).结论包芯结构骨支架在体外表现出良好亲水性和生物相容性,可作为较理想的组织工程骨支架.
목적 제비신형적포심결구골지가,비교기여취유산취간기을산취합물(PLGA)/β-린산삼개(TCP)복합지가적체외생물상용성.방법 지가통과쾌속성형방법제작.친수성통과흡수솔평개；세포재지가상적증식능력통과새서람(MTT)비색법측정,성골능력통과감성린산매활성(ALP)검측평개,생장정황통과소묘전경관측.결과 포심결구조흡수솔위(18.1±0.4)％,교대조조(2.6±0.3)％현저제고(P＜0.01),세포화지가공배양6、10、14d,포심결구조세포활도흡광도(A)치분별위:0.28 ±0.09、0.60 ±0.10、0.76 ±0.10,ALP분별위:124.33±14.22、189.00±19.52、230.00±12.12,대조조A치위:0.19 ±0.09、0.36±0.05、0.46 ±0.12,ALP위:78.33±8.02、105.67±7.77、123.67±20.84;조간차이유통계학의의(P＜0.05).결론 포심결구골지가재체외표현출량호친수성화생물상용성,가작위교이상적조직공정골지가.
Objective To assess whether a wrapped-core structure composite scaffold possesses the biocompatibility in vitro.Methods The wrapped-core structure composite scaffold composed of poly lactic-co-glycolic acid (PLGA)/β-TCP skeleton wrapped with type Ⅰ collagen was manufactured via LDM,and PLGA/β-TCP scaffold was chosen as the control group.SEM was used to study morphology of bone marrow mesenchymal stem cells (BMSCs) on the surface of the original scaffolds.Hydrophilicity was estimated by water absorption ratio.Cell proliferation was estimated by methyl thiazol tetrazolium (MTT) assay and the osteogenic differentiation of BMSCs was estimated by alkaline phosphatase (ALP).Results The water absorption of the original scaffold [(18.1 ± 0.4) ％] was significantly higher (P ＜ 0.01) than that of the control group [(2.6 ±0.3)％].After co-culture of BMSCs for 6,10,and 14 days,the A values of original scaffolds and control groups were 0.28 ± 0.09,0.60 ± 0.10,0.76 ± 0.10,and 0.19 ± 0.09,0.36 ±0.05,0.46 ± 0.12,respectively; the activities of ALP were 124.33 ± 14.22,189.00 ± 19.52,230.00 ± 12.12,and 78.33 ± 8.02,105.67 ± 7.77,123.67 ± 20.84,respectively.There was significant difference between the two groups (P ＜ 0.05).Conclusion The wrapped-core structure composite scaffold possesses admirable biocompatibility and is expected to be of useful value in bone tissue engineering.