CAJ | 학술논문

目的：组织工程技术重建膀胱后对上尿路结构及功能影响的分析和评估。方法将8只雄性比格犬随机分为两组：假手术对照组（A组）4只，胶原支架修复组（B组）4只，建立B组膀胱缺损动物模型，利用胶原支架材料修复重建膀胱。两组于修复前及修复后23周行肾功能相关生化指标测定以及肾脏超声检查，术后24周行标本大体形态观察及组织学检查。结果肾脏超声检查两组动物肾脏形态、血流信号正常。大体观察两组实验动物肾脏及输尿管无结石、新生物。两组肾功能相关生化指标比较，差异无统计学意义（P＞0．05）。组织学检查提示组织结构完整，两组肾单位无明显病理改变。结论利用胶原支架材料重建膀胱后对上尿路结构及功能未产生不良影响，这种组织工程方法具有较好的可行性。
목적：조직공정기술중건방광후대상뇨로결구급공능영향적분석화평고。방법장8지웅성비격견수궤분위량조：가수술대조조（A조）4지，효원지가수복조（B조）4지，건립B조방광결손동물모형，이용효원지가재료수복중건방광。량조우수복전급수복후23주행신공능상관생화지표측정이급신장초성검사，술후24주행표본대체형태관찰급조직학검사。결과신장초성검사량조동물신장형태、혈류신호정상。대체관찰량조실험동물신장급수뇨관무결석、신생물。량조신공능상관생화지표비교，차이무통계학의의（P＞0．05）。조직학검사제시조직결구완정，량조신단위무명현병리개변。결론이용효원지가재료중건방광후대상뇨로결구급공능미산생불량영향，저충조직공정방법구유교호적가행성。
Objective To analyze and evaluate the effects of the tissue engineering bladder reconstruction on the upper urinary tract structure and function.Methods The 8 male beagles were randomly divided into the two groups:sham-operation group (group A,n=4)and the collagen scaffold repair group (group B,n=4).The bladder defect animal model was established in the group B by using the collagen scaffold materials to repair the bladder.The renal function related biochemical indicators were detec-ted and the renal Doppler ultrasonic examination was performed in each group before repair and in 23 weeks after repair.The speci-mens from the two groups were performed the gross morphology observation and the histology examination on postoperative 24 weeks.Results The renal Doppler ultrasound examination showed the normal kidney morphology and normal blood flow signal.In the general observation,no calculi and neoplasm were found in the kidney and ureter of the experimental dogs.The renal function related biochemical indicators had no statistically significant differences between the two groups(P>0.05).The histological exami-nation indicated that the organization structure was integrity,the nephrons in each group had no obvious pathological changes.Con-clusion Using the collagen scaffold materials to reconstruct the canine bladder has no adverse influence on the upper urinary tract structure and function,this tissue engineering approach has good feasibility.