CAJ | 학술논문

目的：通过莲心碱作用于膀胱癌细胞T24，研究其对膀胱癌细胞T24增殖的影响。方法使用不同浓度的莲心碱处理膀胱癌细胞T24，通过CCK-8实验及克隆形成实验，检测莲心碱对细胞增殖的影响；对细胞进行 PI染色后，使用流式细胞仪，检测莲心碱对T24细胞周期的影响；通过实时定量PCR，检测莲心碱作用后T24细胞的 p21基因mRNA的改变。结果莲心碱对膀胱癌细胞T24增殖有明显的抑制作用，各剂量组（1．5625、3．1250、6．2500、12．5000、25．0000μg/mL）与甲醇对照组差异有统计学意义（P＜0．05），并呈剂量依赖性；细胞周期检测结果显示，莲心碱将 T24细胞阻滞在 S期；实时定量 PCR检测结果显示，莲心碱提高T24细胞 p21基因的mRNA。结论莲心碱对膀胱癌细胞T24具有增殖抑制并将其阻滞在 S期，该作用机制可能与 p21表达上调有关。
목적：통과연심감작용우방광암세포T24，연구기대방광암세포T24증식적영향。방법사용불동농도적연심감처리방광암세포T24，통과CCK-8실험급극륭형성실험，검측연심감대세포증식적영향；대세포진행 PI염색후，사용류식세포의，검측연심감대T24세포주기적영향；통과실시정량PCR，검측연심감작용후T24세포적 p21기인mRNA적개변。결과연심감대방광암세포T24증식유명현적억제작용，각제량조（1．5625、3．1250、6．2500、12．5000、25．0000μg/mL）여갑순대조조차이유통계학의의（P＜0．05），병정제량의뢰성；세포주기검측결과현시，연심감장 T24세포조체재 S기；실시정량 PCR검측결과현시，연심감제고T24세포 p21기인적mRNA。결론연심감대방광암세포T24구유증식억제병장기조체재 S기，해작용궤제가능여 p21표체상조유관。
Objective To study the effect of liensinine on the proliferation of human bladder cancer T24 cells.Methods T24 cells were treated with different concentrations of liensinine.Its influence on the cell proliferation was detected by the CCK-8 exper-iment and the clonogenic experiment.After staining of T24 cells,the influence of liensinine on the cell cycle was examined by the flow cytometry.The mRNA change of p2 1 gene was determined by real-time quantitative PCR.Results Compared with the con-trol,liensinine significantly inhibited the proliferation of T24 cells in different doses groups(1.562 5,3.125 0,6.250 0,12.500 0, 25.000 0μg/mL),the differences had statistical significance and showed the dose-dependence;the cell cycle detection results re-vealed that liensinine arrested the T24 cells at the S phase;the real-time quantitative PCR detection results showed that liensinine increased mRNA of p21 gene in T24 cells.Conclusion Liensinine inhibits the proliferation of T24 bladder cancer cells and arrests the T24 cells at S phase,its mechanism may be related with the upregulation of p21 expression.