重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2014年
26期
3452-3454
,共3页
端粒 ,末端转移酶%p53正向凋亡调控因子%卵巢肿瘤%腺病毒科
耑粒 ,末耑轉移酶%p53正嚮凋亡調控因子%卵巢腫瘤%腺病毒科
단립 ,말단전이매%p53정향조망조공인자%란소종류%선병독과
telomerase%P53 up-regulate modulator of apoptosis%ovarian neoplasms%adenoviridae
目的：探讨卵巢癌COC1细胞中大量表达p53正向凋亡调控因子（PUMA）在抑制COC1细胞中的作用。方法采用人端粒酶逆转录酶（hTERT）启动子调控的PUMA基因腺病毒载体（Ad-hTERT-PUMA）转染卵巢癌COC1细胞，通过细胞活性检测试剂盒和凋亡试剂盒研究COC1细胞的生长情况，同时分析表达PUMA的水平。结果转染Ad-hTERT-PUMA后，PU-MA大量表达，抑制COC1细胞生长，促进细胞凋亡。结论Ad-hTERT-PUMA成功转染卵巢癌COC1细胞并大量表达，起到了抑制卵巢癌细胞增长的作用。
目的：探討卵巢癌COC1細胞中大量錶達p53正嚮凋亡調控因子（PUMA）在抑製COC1細胞中的作用。方法採用人耑粒酶逆轉錄酶（hTERT）啟動子調控的PUMA基因腺病毒載體（Ad-hTERT-PUMA）轉染卵巢癌COC1細胞，通過細胞活性檢測試劑盒和凋亡試劑盒研究COC1細胞的生長情況，同時分析錶達PUMA的水平。結果轉染Ad-hTERT-PUMA後，PU-MA大量錶達，抑製COC1細胞生長，促進細胞凋亡。結論Ad-hTERT-PUMA成功轉染卵巢癌COC1細胞併大量錶達，起到瞭抑製卵巢癌細胞增長的作用。
목적：탐토란소암COC1세포중대량표체p53정향조망조공인자（PUMA）재억제COC1세포중적작용。방법채용인단립매역전록매（hTERT）계동자조공적PUMA기인선병독재체（Ad-hTERT-PUMA）전염란소암COC1세포，통과세포활성검측시제합화조망시제합연구COC1세포적생장정황，동시분석표체PUMA적수평。결과전염Ad-hTERT-PUMA후，PU-MA대량표체，억제COC1세포생장，촉진세포조망。결론Ad-hTERT-PUMA성공전염란소암COC1세포병대량표체，기도료억제란소암세포증장적작용。
Objective To over-express the P53 upregulated modulator of apoptosis(PUMA) in ovarian cancer COC1 cells for playing the role to restrain the cell growth .Methods Adenovirus vector (Ad)-human telomerase reverse transcriptase promoter (hTERT)-PUMA was transfected into ovarian cancer COC1 cells .Then the growth of COC1 cells was studied by CCK-8 and the apoptosis kit and the level of PUMA was analyzed .Results After transfection of Ad-hTERT-PUMA ,PUMA was over-expressed in COC1 cells ,which restrained the cells growth and promoted the cell apoptosis .Conclusion Ad-hTERT-PUMA is successfully transfected into ovarian cancer COC1 cells and PUMA is over-expressed to play the role for restraining the cells growth .
