CAJ | 학술논문

目的：探讨虾青素对体外氧化应激诱导的人外周血内皮祖细胞凋亡的影响及机制。方法体外培养人外周血单核细胞源的内皮祖细胞，分为对照组、模型组（叔丁基过氧化氢100μmol/L ）、虾青素＋叔丁基过氧化氢组[虾青素0．10、1．00、10．00 nmol/L预处理24 h后，分别使用叔丁基过氧化氢溶液（100μmol/L）连续培养6 h]。细胞存活率采用MTT法检测；细胞凋亡率采用DAPI染细胞检测；DCFH-DA法检测细胞内活性氧（reactive oxygen species ，ROS）水平；JC-1法测定线粒体膜电位。结果与对照组比较，叔丁基过氧化氢（100μmol/L）能明显的造成内皮祖细胞的凋亡（P＜0．05）。虾青素可降低叔丁基过氧化氢引起的内皮祖细胞凋亡，表现为细胞凋亡率减少（P＜0．05），线粒体膜电位增加。结论虾青素对叔丁基过氧化氢诱导的内皮祖细胞凋亡具有保护作用，其机制可能与保护线粒体功能有关。
목적：탐토하청소대체외양화응격유도적인외주혈내피조세포조망적영향급궤제。방법체외배양인외주혈단핵세포원적내피조세포，분위대조조、모형조（숙정기과양화경100μmol/L ）、하청소＋숙정기과양화경조[하청소0．10、1．00、10．00 nmol/L예처리24 h후，분별사용숙정기과양화경용액（100μmol/L）련속배양6 h]。세포존활솔채용MTT법검측；세포조망솔채용DAPI염세포검측；DCFH-DA법검측세포내활성양（reactive oxygen species ，ROS）수평；JC-1법측정선립체막전위。결과여대조조비교，숙정기과양화경（100μmol/L）능명현적조성내피조세포적조망（P＜0．05）。하청소가강저숙정기과양화경인기적내피조세포조망，표현위세포조망솔감소（P＜0．05），선립체막전위증가。결론하청소대숙정기과양화경유도적내피조세포조망구유보호작용，기궤제가능여보호선립체공능유관。
Objective To investigate the effect of astaxanthin on the peripheral blood endothelial progenitor cells (EPCs) apop-tosis induced by oxidative stress in vitro and to explore its underlying mechanism .Methods Human peripheral blood EPCs were in vitro cultured and divided into the control group ,model group with 100 μmol/L tert-butyl hydroperoxide(tBHP) and the astaxan-thin plus tBHP group(with 0 .10 ,1 .00 ,10 .00 nmol/L astaxanthin pretreatment for 24 h ,then adding the final concentration of 100μmol/L tBHP for 6 h continuous culture) .The cell viability was measured by the MTT method .The level of reactive oxygen spe-cies (ROS) was determined by the DCFH-DA method .The changes of mitochondrial membrane potential(MMP) and the apoptosis ratio were detected by the JC-1 method and the DAPI method ,respectively .Results Compared with control group ,100μmol/L tB-HP could obviously caused the apoptosis of EPCs(P<0 .05) ,while astaxanthin could decrease tBHP induced apoptosis ,which man-ifested by the decrease of the apoptosis ratio (P<0 .05) and MMP increase .Conclusion Astaxanthin has the protective effect on the apoptosis of EPCs ,its mechanism may be related with the protection of the mitochondrial function .