CAJ | 학술논문

目的：探讨中性粒细胞弹性蛋白酶（NE）抑制剂对颅脑创伤（TBI）大鼠血脑屏障（BBB）通透性改变和脑水肿的影响。方法将99只大鼠分为对照组、TBI组和干预组（分为6、24、48、72、168 h亚组），复制大鼠液压打击模型，干预组给予西维来司钠，分别测定各组脑组织NE的浓度、BBB通透性和脑组织含水率，并进行比较分析。结果 TBI组和干预组大鼠脑组织NE浓度、BBB通透性和脑组织含水率在各时间点都高于对照组，干预组的NE浓度在24、48、72、168 h低于TBI组，BBB通透性和脑组织含水率在48、72、168 h低于TBI组（P＜0．05）。结论西维来司钠能抑制TBI大鼠脑组织NE的释放，降低BBB的通透性并减轻脑水肿发生，表明西维来司钠对大鼠TBI继发性损伤有保护作用。
목적：탐토중성립세포탄성단백매（NE）억제제대로뇌창상（TBI）대서혈뇌병장（BBB）통투성개변화뇌수종적영향。방법장99지대서분위대조조、TBI조화간예조（분위6、24、48、72、168 h아조），복제대서액압타격모형，간예조급여서유래사납，분별측정각조뇌조직NE적농도、BBB통투성화뇌조직함수솔，병진행비교분석。결과 TBI조화간예조대서뇌조직NE농도、BBB통투성화뇌조직함수솔재각시간점도고우대조조，간예조적NE농도재24、48、72、168 h저우TBI조，BBB통투성화뇌조직함수솔재48、72、168 h저우TBI조（P＜0．05）。결론서유래사납능억제TBI대서뇌조직NE적석방，강저BBB적통투성병감경뇌수종발생，표명서유래사납대대서TBI계발성손상유보호작용。
Objective To investigate the effect of neutrophil elastase (NE) inhibitor on the blood-brain barrier(BBB) permeabili-ty and hydrocephalus in rats with traumatic brain injury (TBI) .Methods 99 SD rats were randomly divided into the control group , the TBI group and the intervention group(dividing into 5 sub-groups:6 ,24 ,48 ,72 ,168 h) .The hydraulic impact model of rats was duplicated .Sivelestat sodium was given in the intervention group .The NE concentration in the brain tissue ,BBB permeability and brain water content were detected in each group and performed the comparative analysis .Results The NE concentration in the brain tissue ,BBB permeability and brain water content at each timepoint in the TBI group and the intervention groups were higher than those in the control group .The NE concentration at 24 ,48 ,72 ,168 h in the intervention group was lower than that in the TBI group .The BBB permeability and the brain water content at 48 ,72 ,168 h in the intervention group were lower than those in the TBI group(P<0 .05) .Conclusion Sivelestat sodium can inhibit the NE release in TBI rat brain tissue ,reduce the BBB permeability and the occurrence of hydrocephalus ,which indicating that sivelestat sodium has the protective effect on TBI secondary lesion in rat .