CAJ | 학술논문

重庆汉族人群HLA-A、B、DRB1高分辨等位基因多态性研究
중경한족인군HLA-A、B、DRB1고분변등위기인다태성연구
Study on HLA-A,B,DRB1 high-resolution alleles polymorphism in Chongqing Han population

万方数据

重庆医学重慶醫學 중경의학
CHONGQING MEDICAL JOURNAL
2014年 26期 3455-3457,3460 ,共4页

王芳%廖群%黄霞%张涛%朱素敏%廖红梅%李小红%程磊%谭茜茜%黄红莉%宋正利

왕방%료군%황하%장도%주소민%료홍매%리소홍%정뢰%담천천%황홍리%송정리

HLA抗原%高分辨基因分型%基因频率%多态性%重庆 HLA抗原%高分辨基因分型%基因頻率%多態性%重慶
HLA항원%고분변기인분형%기인빈솔%다태성%중경
HLA antigens%high-resolution typing%gene frequency%polymorphism%Chongqing

目的：分析重庆地区汉族人群 HLA-A、B、DRB1在高分辨基因分型水平上多态性及分布特征。方法采用聚合酶链反应-序列特异性寡核苷酸探针（PCR-SSOP）基因分型以及基因序列分型（SBT ）技术，对重庆地区2067例无关供者的HLA-A、B、DRB1位点上的等位基因进行高分辨分型，直接计数法计算等位基因频率，应用Arlerquin3．1软件进行Hardy-Wein-berg平衡检验。结果共检出了168种 HLA高分辨等位基因。其中 HLA-A位点42种，频率大于0．05有 A倡11：01，A倡24：02，A倡02：07，A倡02：01，A倡33：03；HLA-B位点81种，频率大于0．05有B倡46：01，B倡40：01，B倡58：01，B倡13：01，B倡15：02；HLA-DR位点45种，频率大于0．05有 DRB1倡09：01，DRB1倡15：01，DRB1倡12：02，DRB1倡08：03，DRB1倡11：01。结论获得了重庆地区汉族人群 HLA-A、B、DRB1高分辨等位基因频率数据，为人类学、法医学、移植配型及疾病相关性等研究提供可靠的参考数据。
목적：분석중경지구한족인군 HLA-A、B、DRB1재고분변기인분형수평상다태성급분포특정。방법채용취합매련반응-서렬특이성과핵감산탐침（PCR-SSOP）기인분형이급기인서렬분형（SBT ）기술，대중경지구2067례무관공자적HLA-A、B、DRB1위점상적등위기인진행고분변분형，직접계수법계산등위기인빈솔，응용Arlerquin3．1연건진행Hardy-Wein-berg평형검험。결과공검출료168충 HLA고분변등위기인。기중 HLA-A위점42충，빈솔대우0．05유 A창11：01，A창24：02，A창02：07，A창02：01，A창33：03；HLA-B위점81충，빈솔대우0．05유B창46：01，B창40：01，B창58：01，B창13：01，B창15：02；HLA-DR위점45충，빈솔대우0．05유 DRB1창09：01，DRB1창15：01，DRB1창12：02，DRB1창08：03，DRB1창11：01。결론획득료중경지구한족인군 HLA-A、B、DRB1고분변등위기인빈솔수거，위인류학、법의학、이식배형급질병상관성등연구제공가고적삼고수거。
Objective To analyze the HLA-A ,B and DRB1 alleles high-resolution polymorphism in Chongqing Han population . Methods The PCR-SSOP and PCR-SBT methods were applied for the HLA high-resolution genotyping of 2 067 unrelated healthy donors in the registry of Chongqing branch of Chinese National Marrow Donor Program (CMDP) .The allele frequencies of HLA-A , B and DRB1 were estimated by the direct counting method and the Hardy-Weinberg equilibrium inspection was performed by using the Arlequin software 3 .1 .Results 168 high-resolution alleles were detected out ,in which 42 alleles of A*11 :01 ,A*24:02 ,A*02:07 ,A*02 :01 and A*33:03 at the HLA-A locus were observed with the frequencies greater than 0 .05 ;81 alleles were detected at HLA-B locus ,including B*46 :01 ,B*40:01 ,B*58 :01 ,B*13 :01 and B*15 :02 with the frequencies greater than 0 .05 ;45 al-leles of DRB1*09:01 ,DRB1*15 :01 ,DRB1*12 :02 ,DRB1*08 :03 and DRB1*11 :01 at the HLA-DR locus were observed with the frequencies greater than 0 .05 .Conclusion The data of the HLA-A ,B and DRB1allelic frequencies at high-resolution level in Chongqing Han population are obtained ,which provides the reliable reference data for the studies of anthropology ,forensic medi-cine ,transplantation matching and disease association .