重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2014年
26期
3449-3451,3454
,共4页
戴玲丽%梅同华%张明川
戴玲麗%梅同華%張明川
대령려%매동화%장명천
程序化死亡因子5%顺铂%Bcl-2%Survivin%细胞凋亡
程序化死亡因子5%順鉑%Bcl-2%Survivin%細胞凋亡
정서화사망인자5%순박%Bcl-2%Survivin%세포조망
programmed cell death 5%cisplatin%Bcl-2%Survivin%cell apoptosis
目的：观察程序化死亡因子5（PDCD5）基因联合顺铂诱导人肺腺癌A549细胞凋亡的作用，探索其可能机制。方法利用脂质体瞬时转染PDCD5重组质粒入 A549细胞，RT-PCR检测其转染效率。Western blot检测转染后 PDCD5、Bcl-2和Survivin蛋白表达情况。M T T法及流式细胞仪检测PDCD5单药和联合顺铂后的细胞凋亡情况。结果 PDCD5重组质粒成功转染入A549细胞，Western bolt结果显示转染重组质粒组中PDCD5蛋白表达高于空白对照组及转染空质粒组，Bcl-2和Survivin蛋白则低于空白对照组及转染空质粒组，差异有统计学意义（P＜0．05）。MTT及流式细胞仪检测显示转染重组质粒组较空白对照组及转染空质粒组细胞凋亡率高（ P＜0．05）。结论 PDCD5基因可以促进顺铂诱导的A549细胞凋亡。
目的：觀察程序化死亡因子5（PDCD5）基因聯閤順鉑誘導人肺腺癌A549細胞凋亡的作用，探索其可能機製。方法利用脂質體瞬時轉染PDCD5重組質粒入 A549細胞，RT-PCR檢測其轉染效率。Western blot檢測轉染後 PDCD5、Bcl-2和Survivin蛋白錶達情況。M T T法及流式細胞儀檢測PDCD5單藥和聯閤順鉑後的細胞凋亡情況。結果 PDCD5重組質粒成功轉染入A549細胞，Western bolt結果顯示轉染重組質粒組中PDCD5蛋白錶達高于空白對照組及轉染空質粒組，Bcl-2和Survivin蛋白則低于空白對照組及轉染空質粒組，差異有統計學意義（P＜0．05）。MTT及流式細胞儀檢測顯示轉染重組質粒組較空白對照組及轉染空質粒組細胞凋亡率高（ P＜0．05）。結論 PDCD5基因可以促進順鉑誘導的A549細胞凋亡。
목적：관찰정서화사망인자5（PDCD5）기인연합순박유도인폐선암A549세포조망적작용，탐색기가능궤제。방법이용지질체순시전염PDCD5중조질립입 A549세포，RT-PCR검측기전염효솔。Western blot검측전염후 PDCD5、Bcl-2화Survivin단백표체정황。M T T법급류식세포의검측PDCD5단약화연합순박후적세포조망정황。결과 PDCD5중조질립성공전염입A549세포，Western bolt결과현시전염중조질립조중PDCD5단백표체고우공백대조조급전염공질립조，Bcl-2화Survivin단백칙저우공백대조조급전염공질립조，차이유통계학의의（P＜0．05）。MTT급류식세포의검측현시전염중조질립조교공백대조조급전염공질립조세포조망솔고（ P＜0．05）。결론 PDCD5기인가이촉진순박유도적A549세포조망。
Objective To observe the role of programmed cell death 5(PDCD5) gene combined with cisplatin for inducing the ap-optosis of human lung adenocarcinoma A549 cells and to investigate its possible mechanism .Methods The PDCD5 recombinant plas-mid was transiently transfected into A549 cells by lipofectamine .Its transfection efficiency was detected by RT-PCR .The expressions of trasfected PDCD5 protein ,Bcl-2 and Survivin protein were examined by Western bolt .The apoptosis of A549 cells by single PDCD5 and its combination with cisplatin was measured by the MTT method and the flow cytometry .Results PDCD5 recombinant plasmid was transfect into A549 cells successfully .The Western blot results showed that the expression of PDCD5 protein in the transfection recombinant plasmid group was higher than that in the blank control group and the transfection empty plasmid group ,while the expres-sion of Bcl-2 and Survivin protein was lower than that in the other two groups ,the difference was statistically significant (P<0 .05) . The MTT and flow cytometry results demonstrated that the cell apoptosis rate in the transfection recombinant plasmid group was higher than that in the blank control group and the transfection empty plasmid group (P<0 .05) .Conclusion PDCD5 gene may pro-mote cisplatin induced A549 cells apoptosis .
