重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2014年
26期
3409-3411,3415
,共4页
林秋香%周冬生%白纪红%梁志清%赵雪%王雪雯
林鞦香%週鼕生%白紀紅%樑誌清%趙雪%王雪雯
림추향%주동생%백기홍%량지청%조설%왕설문
黄酮类%银杏%植物提取物%脂肪肝%肿瘤坏死因子α%核因子-κBp65
黃酮類%銀杏%植物提取物%脂肪肝%腫瘤壞死因子α%覈因子-κBp65
황동류%은행%식물제취물%지방간%종류배사인자α%핵인자-κBp65
flavonoid%gingko biloba%plant extracts%fatty liver%tumor necrosis factor-alpha%nuclear factor-κBp65
目的：观察不同剂量银杏黄酮对非酒精性脂肪肝病（NAFLD）小鼠肝脏核因子-κBp65（NF-κBp65）和肿瘤坏死因子（T N F-α）的影响。方法采用高脂饲料喂养4周，构建小鼠N A FLD模型。小鼠随机分为正常对照组，高脂模型组，银杏黄酮高、中、低剂量治疗组。银杏黄酮高、中、低剂量组分别予不同剂量银杏黄酮灌胃，正常对照组和高脂模型组予生理盐水灌胃。治疗12周后处死小鼠检测肝组织 NF-κBp65表达，血清 TNF-α、三酰甘油（TG）水平。结果与正常对照组比较，高脂模型组 NF-κBp65、TNF-α、TG水平升高（P＜0．05）。与高脂模型组比较，银杏黄酮高、中、低剂量组 NF-κBp65、TNF-α、TG水平降低（P＜0．05），且剂量越高降低越明显。结论银杏黄酮可减少NF-κBp65及TNF-α、TG的产生，从而对NAFLD起到一定治疗作用。
目的：觀察不同劑量銀杏黃酮對非酒精性脂肪肝病（NAFLD）小鼠肝髒覈因子-κBp65（NF-κBp65）和腫瘤壞死因子（T N F-α）的影響。方法採用高脂飼料餵養4週，構建小鼠N A FLD模型。小鼠隨機分為正常對照組，高脂模型組，銀杏黃酮高、中、低劑量治療組。銀杏黃酮高、中、低劑量組分彆予不同劑量銀杏黃酮灌胃，正常對照組和高脂模型組予生理鹽水灌胃。治療12週後處死小鼠檢測肝組織 NF-κBp65錶達，血清 TNF-α、三酰甘油（TG）水平。結果與正常對照組比較，高脂模型組 NF-κBp65、TNF-α、TG水平升高（P＜0．05）。與高脂模型組比較，銀杏黃酮高、中、低劑量組 NF-κBp65、TNF-α、TG水平降低（P＜0．05），且劑量越高降低越明顯。結論銀杏黃酮可減少NF-κBp65及TNF-α、TG的產生，從而對NAFLD起到一定治療作用。
목적：관찰불동제량은행황동대비주정성지방간병（NAFLD）소서간장핵인자-κBp65（NF-κBp65）화종류배사인자（T N F-α）적영향。방법채용고지사료위양4주，구건소서N A FLD모형。소서수궤분위정상대조조，고지모형조，은행황동고、중、저제량치료조。은행황동고、중、저제량조분별여불동제량은행황동관위，정상대조조화고지모형조여생리염수관위。치료12주후처사소서검측간조직 NF-κBp65표체，혈청 TNF-α、삼선감유（TG）수평。결과여정상대조조비교，고지모형조 NF-κBp65、TNF-α、TG수평승고（P＜0．05）。여고지모형조비교，은행황동고、중、저제량조 NF-κBp65、TNF-α、TG수평강저（P＜0．05），차제량월고강저월명현。결론은행황동가감소NF-κBp65급TNF-α、TG적산생，종이대NAFLD기도일정치료작용。
Objective To investigate the effects of different doses of gingko flavonoid on the expression of nuclear factor-κBp65 (NF-κBp65) and tumor necrosis factor-α(TNF-α) in mice with non-alcoholic fatty liver disease(NAFLD) .Methods The mice mod-el of NAFLD was constructed by the high fat diet for 4 weeks .The mice were randomly divided into the control group ,high fat model group ,high ,middle and low doses of gingko flavonoid groups .High ,middle and low doses of gingko flavonoid groups were respectively gavaged with the different doses of gingko flavonoid .The normal control group and the high fat model group were ga-vaged with normal saline .After 12 weeks ,the mice were killed for taking liver tissue and detecting NF-κBp65 expressions ,and ser-um TNF-αand triacylglycerol(TG) levels were detected .Results Compared with the normal control group ,the levels of NF-κBp65 ,TNF-αand TG in the high fat model group were increased(P<0 .05) .Compared with the high fat model group ,the levels of NF-κBp65 ,TNF-αand TG in the high ,middle and low doses of gingko flavonoid groups were decreased (P<0 .05) ,moreover the higher the dose ,the more obvious the decrease .Conclusion Gingko flavonoid may play certain role in the treatment of NAFLD by reducing the generation of NF-κBp65 ,TNF-αand TG .
