靶向bcr/abl融合基因的RNAi对慢性粒细胞白血病K562增殖活性的影响
파향bcr/abl융합기인적RNAi대만성립세포백혈병K562증식활성적영향
The Effect of RNAi Targeting bcr/abl Fusion Gene on the Proliferation Activity of the Chronic Myelogenous Leukemia K562
  • 万方数据
    热带生物学报 열대생물학보
    JOURNAL OF SOUTH CHINA UNIVERSITY OF TROPICAL AGRICULTURE
    2014年 1期 25-29 ,共5页

    潘彦鹏%曹献英%武昭颖%常志杰%王银银%田博%郝新宝

    반언붕%조헌영%무소영%상지걸%왕은은%전박%학신보

    慢病毒%bcr/abl基因,P210bcr/abl%增殖 慢病毒%bcr/abl基因,P210bcr/abl%增殖
    만병독%bcr/abl기인,P210bcr/abl%증식
    lentivirus%bcr/abl gene%P210bcr/abl%proliferation
    构建含bcr/abl RNAi慢病毒重组质粒载体并包装病毒,转染K562细胞,通过Real-time PCR及Western blotting验证干扰效应.采用CCK-8法、软琼脂细胞集落形成实验检测RNAi对K562细胞的增殖影响作用.结果表明,慢病毒介导bcr/abl基因RNAi明显下调K562细胞中bcr/abl mRNA及P210bcr/abl融合蛋白,显著抑制了K562细胞的增殖,这说明靶向bcr/abl融合基因的RNAi有望成为治疗慢性粒细胞白血病的有效方法.
    구건함bcr/abl RNAi만병독중조질립재체병포장병독,전염K562세포,통과Real-time PCR급Western blotting험증간우효응.채용CCK-8법、연경지세포집락형성실험검측RNAi대K562세포적증식영향작용.결과표명,만병독개도bcr/abl기인RNAi명현하조K562세포중bcr/abl mRNA급P210bcr/abl융합단백,현저억제료K562세포적증식,저설명파향bcr/abl융합기인적RNAi유망성위치료만성립세포백혈병적유효방법.
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