玉林师范学院学报
玉林師範學院學報
옥림사범학원학보
Journal of Yulin Teachers College
2014年
2期
59-63,67
,共6页
互叶白千层%愈伤组织%诱导%分化%伸长与生根
互葉白韆層%愈傷組織%誘導%分化%伸長與生根
호협백천층%유상조직%유도%분화%신장여생근
Melaleuca alternifolia%callus%induction%differentiation%elongation and rooting
本文报导了互叶白千层从无菌种子苗叶片诱导愈伤组织到植株再生的研究结果.实验表明：以继代代数不同的无菌苗叶片为诱导材料，在MS基本培养基中添加TDZ0.4mg/L+2,4-D0.4mg/L的第五代继代苗诱导效果较好，愈伤组织诱导率达100%.在互叶白千层愈伤组织分化阶段，在MS基本培养基中，激素组合筛选中以添加6-BA2.0mg/L+IAA2.5mg/L的分化效果较好，分化率高达94.53%.在分化苗伸长生根阶段，以MS+6-BA2.0mg/L+NAA0.8mg/L +AC 0.1%的效果较为显著，苗平均高度9.4cm，苗最壮，根粗且壮.
本文報導瞭互葉白韆層從無菌種子苗葉片誘導愈傷組織到植株再生的研究結果.實驗錶明：以繼代代數不同的無菌苗葉片為誘導材料，在MS基本培養基中添加TDZ0.4mg/L+2,4-D0.4mg/L的第五代繼代苗誘導效果較好，愈傷組織誘導率達100%.在互葉白韆層愈傷組織分化階段，在MS基本培養基中，激素組閤篩選中以添加6-BA2.0mg/L+IAA2.5mg/L的分化效果較好，分化率高達94.53%.在分化苗伸長生根階段，以MS+6-BA2.0mg/L+NAA0.8mg/L +AC 0.1%的效果較為顯著，苗平均高度9.4cm，苗最壯，根粗且壯.
본문보도료호협백천층종무균충자묘협편유도유상조직도식주재생적연구결과.실험표명：이계대대수불동적무균묘협편위유도재료，재MS기본배양기중첨가TDZ0.4mg/L+2,4-D0.4mg/L적제오대계대묘유도효과교호，유상조직유도솔체100%.재호협백천층유상조직분화계단，재MS기본배양기중，격소조합사선중이첨가6-BA2.0mg/L+IAA2.5mg/L적분화효과교호，분화솔고체94.53%.재분화묘신장생근계단，이MS+6-BA2.0mg/L+NAA0.8mg/L +AC 0.1%적효과교위현저，묘평균고도9.4cm，묘최장，근조차장.
In this study, the callus induction of aseptic seedlings and the plantlet regeneration of Melaleuca alternifolia were reported. The results showed that the optimization callus induction media was MS medium add TDZ0.4 mg/L+2,4-D0.4mg/L used the fifth generation aseptic seedling and the induction rate achieved 100%. In the stage of callus differentiation, the best differential medium was MS medium supplied with 6-BA2.0mg/L+IAA2.5mg/L and differentiation rate of callus reached 94.53%. The best medium for regenerated plantlets rooting was MS + 6-BA2.0mg/L+NAA0.8mg/L +AC 0.1% and the average of seedling height reached 9.4cm. The seedlings and roots were strongest than those in the other medium.
