CAJ | 학술논문

对采自襄城县连作1a-8a的烟田土壤的微生物数量、细菌多样性和土壤中的蔗糖酶、淀粉酶、纤维素酶和进行了测定。结果表明：随着连作年限的增加，土壤细菌和放线菌数量呈明显下降趋势，而真菌数量变化表现出一定的滞后性，呈现升高-降低-升高-降低的波动；土壤细菌的Shannon指数、 Simpson指数和McIntosh多样性指数整体呈下降趋势，仅在连作5a时出现一定的上升波动，与土壤细菌的分离培养结果相吻合；土壤蔗糖酶和淀粉酶活性先升高后降低，连作2a时达到最大值，当连作年限﹥3a时，酶活性变化不大，都处于较低水平；而土壤纤维素酶活性在连作1a -3a时便剧烈下降，连作年限﹥2a时，酶活性维持在较低的水平。烟草长期连作对土壤微生物的多样性和土壤酶活性的影响，不利于土壤生态系统的健康发展。图5，表1，参28。
대채자양성현련작1a-8a적연전토양적미생물수량、세균다양성화토양중적자당매、정분매、섬유소매화진행료측정。결과표명：수착련작년한적증가，토양세균화방선균수량정명현하강추세，이진균수량변화표현출일정적체후성，정현승고-강저-승고-강저적파동；토양세균적Shannon지수、 Simpson지수화McIntosh다양성지수정체정하강추세，부재련작5a시출현일정적상승파동，여토양세균적분리배양결과상문합；토양자당매화정분매활성선승고후강저，련작2a시체도최대치，당련작년한﹥3a시，매활성변화불대，도처우교저수평；이토양섬유소매활성재련작1a -3a시편극렬하강，련작년한﹥2a시，매활성유지재교저적수평。연초장기련작대토양미생물적다양성화토양매활성적영향，불리우토양생태계통적건강발전。도5，표1，삼28。
Soil samples were collected from continuous cropping 1a-8a(except 7 year) tobacco fields in Xiangcheng county ,and the a-mount of soil microbial,bacteria diversity,activities of sucrase,amylase,pectase cellulase were determined.Results showed that with the increasing of continuous cropping year ,the number of soil bacteria and actinomycetes was going down ,while the number of soil fungus kept the trend of “up-down-up-down”.The bacterial diversity index as estimated by DGGE banding patterns ,e.g.Shannon ,Simp-son and McIntosh index had the same trend of falling down ,but had a slight rise in the fifth continuous cropping year that consistenting with the results of soil bacteria isolation.The changes of invertase and amylase activity revealed increased at the begaining and reached a peak in second continuous cropping year and then decreased.When the continuous cropping year is greater than 3 years,the invertase and amylase activities changed a little and then kept at a lower level.Meanwhile,the cellulase activity declined violently within 1-3 continuous cropping years ,and kept at a lower levels when the continuous cropping term more than 2 years.The impact of tobacco con-tinuous cropping on soil microbial diversity and soil enzyme activities csused the disadvantage of healthy soil ecosystem development .