CAJ | 학술논문

目的：预测并鉴定粉尘螨3类变应原（Der f 3）中的B细胞线性表位。方法运用生物信息学技术，从抗原性、表面易接近性、柔韧性、亲水性、β转角等5个方面在线预测Der f 3的B细胞线性抗原表位。同时，采用人工合成法合成相应B细胞表位肽分别与由12份螨性过敏性哮喘患者血清组成的3个血清池共同作用，明确其中的强阳性表位肽序列。结果成功预测了Der f 3中的8个B细胞表位肽。其中，在过敏性患者血清与变应原特异性IgE结合试验中确认了33KAKAG-DCP40、86HASGGEKIQVAEIYQHENYDSMTID110、118LKTPMTLDQTNAKPVPLPPQGSDVKVG144、199DVANGGVDSCQGDSGGPV-VD218和156QEGSYSLP1635个强阳性表位肽序列。结论成功确认了Der f 3变应原中5个B细胞线性表位序列，为尘螨过敏性哮喘的诊断和治疗奠定基础。
목적：예측병감정분진만3류변응원（Der f 3）중적B세포선성표위。방법운용생물신식학기술，종항원성、표면역접근성、유인성、친수성、β전각등5개방면재선예측Der f 3적B세포선성항원표위。동시，채용인공합성법합성상응B세포표위태분별여유12빈만성과민성효천환자혈청조성적3개혈청지공동작용，명학기중적강양성표위태서렬。결과성공예측료Der f 3중적8개B세포표위태。기중，재과민성환자혈청여변응원특이성IgE결합시험중학인료33KAKAG-DCP40、86HASGGEKIQVAEIYQHENYDSMTID110、118LKTPMTLDQTNAKPVPLPPQGSDVKVG144、199DVANGGVDSCQGDSGGPV-VD218화156QEGSYSLP1635개강양성표위태서렬。결론성공학인료Der f 3변응원중5개B세포선성표위서렬，위진만과민성효천적진단화치료전정기출。
Objective To predict and identify the linear B-cell epitopes in the major group 3 allergen derived from Derma-tophagoides farina(Der f 3). Methods The linear B-cell epitopes of Der f 3 allergen were analyzed based on the physicochemi-cal properties of amino acids including antigenicity,surface accessibility,flexibility,hydrophilicity,beta-turn by online bioinfor-matics softwares. The eight predicted peptides of linear B-cell epitopes were artificially synthesized and incubated with three aller-gic serum pools(4 serum samples in each),which were consisted of total 12 serum samples from the allergic individuals,and the strong positive epitopes were selected. Results Eight B-cell epitopes from Der f 3 were predicted successfully. Five of eight B-cell epitopes were identified with strong IgE-binding abilities followed by specific IgE assay. The amino acid sequences of them were following:33KAKAGDCP40, 86HASGGEKIQVAEIYQHENYDSMTID110, 118LKTPMTLDQTNAKPVPLPPQGSDVKVG144, 156QEGSYSLP163 and 199DVANGGVDSCQGDSGGPVVD218. Conclusions Five linear B-cell epitopes of Der f 3 allergen have been identified successfully. This result might provide a basis of the diagnosis and treatment for asthma.