CAJ | 학술논문

目的：探讨CXCR 4、血管内皮生长因子-C( VEGF-C)及受体VEGFR-3在原位灶及淋巴结组织中的表达与宫颈癌转移之间的关系。并探讨这几个因子之间的关系。方法：采用实时荧光定量聚合酶链反应技术( Real Time PCR),对40例宫颈癌病人的原位灶组织中的CXCR 4基因和10例正常宫颈组织中的CXCR 4基因进行定量检测。用免疫组化方法( SP法)检测40例宫颈癌病人的原位灶组织中及淋巴结组织中的VEGF-C及受体VEGFR-3的表达。结果：CXCR 4基因的表达与分化程度、病理类型、年龄无关,与临床分期、淋巴结转移有关。 VEGF-C阳性表达率与宫颈癌分期、分化程度、病理类型、年龄无相关。与淋巴结转移有相关, VEGF-C和VEGFR-3表达有显著相关性(P=0.022)。但VEGFR-3阳性表达率与淋巴结转移无相关。结论：CXCR 4基因和VEGF-C、VEGFR-3两种蛋白表达均与宫颈癌的发生发展相关,VEGF-C和VEGFR-3表达有显著相关性。
목적：탐토CXCR 4、혈관내피생장인자-C( VEGF-C)급수체VEGFR-3재원위조급림파결조직중적표체여궁경암전이지간적관계。병탐토저궤개인자지간적관계。방법：채용실시형광정량취합매련반응기술( Real Time PCR),대40례궁경암병인적원위조조직중적CXCR 4기인화10례정상궁경조직중적CXCR 4기인진행정량검측。용면역조화방법( SP법)검측40례궁경암병인적원위조조직중급림파결조직중적VEGF-C급수체VEGFR-3적표체。결과：CXCR 4기인적표체여분화정도、병리류형、년령무관,여림상분기、림파결전이유관。 VEGF-C양성표체솔여궁경암분기、분화정도、병리류형、년령무상관。여림파결전이유상관, VEGF-C화VEGFR-3표체유현저상관성(P=0.022)。단VEGFR-3양성표체솔여림파결전이무상관。결론：CXCR 4기인화VEGF-C、VEGFR-3량충단백표체균여궁경암적발생발전상관,VEGF-C화VEGFR-3표체유현저상관성。
Objective:To investigate the expression of the chemokine receptor ( CXCR 4 ) , the vascular endothelial growth factor-C( VEGF-C) and the vascular endothelial growth factor receptor-3 (VEGFR-3)in cervical carcinoma and lymph node. Methods: CXCR 4 gene expression in 30 cases of cervical carcinoma and lymph node tissues,and 10 cases of normal cervical tissues by real time poly-merase chain reaction ( real time PCR ) . Immunohistochemical techniques were used to detect the expression of VEGF-C and VEGFR-3 in 30 cases of cervical carcinoma and lymph node tissues. Result:CXCR 4 gene expression and degree of differentiation,histological type,age has nothing to do with the clinical stage and lymph node metastasis. The rate of positive expression of VEGF-C and cervical cancer stage, tumor differentiation, histological type, age no correlation. With lymph node metastasis,VEGF-C and VEGFR-3 expression was significantly correlated(P=0.022). But no correlation VEGFR-3 positive expression rate of lymph node metastasis. Conclusion:CXCR 4 gene and VEGF-C,VEGFR-3 both protein are associated with the development of cervical cancer,VEGF-C and VEGFR-3 expression have a significant correlation.