CAJ | 학술논문

杓兰属植物具有很高的观赏和药用价值，但因被过度采集，已成为濒危植物。菌根真菌是杓兰栽培与繁育是否成功的关键因子。本实验采用rDNA ITS序列扩增技术和MEGA软件对滇西北的云南杓兰和紫点杓兰的菌根真菌进行研究，结果表明：云南杓兰和紫点杓兰的菌根真菌存在显著的物种层面的专一性关系，即菌根真菌与杓兰属植物有较强的共生趋势。分离得到的菌根真菌序列已上传至 NCBI，登录号为 lcl51879、 lcl25153、lcl48033、 lcl38377、 lcl14203、 lcl24557、 lcl52287以及lcl46937。
표란속식물구유흔고적관상화약용개치，단인피과도채집，이성위빈위식물。균근진균시표란재배여번육시부성공적관건인자。본실험채용rDNA ITS서렬확증기술화MEGA연건대전서북적운남표란화자점표란적균근진균진행연구，결과표명：운남표란화자점표란적균근진균존재현저적물충층면적전일성관계，즉균근진균여표란속식물유교강적공생추세。분리득도적균근진균서렬이상전지 NCBI，등록호위 lcl51879、 lcl25153、lcl48033、 lcl38377、 lcl14203、 lcl24557、 lcl52287이급lcl46937。
Wild lady’s slipper orchids (Cypripedium calceolus) as an ornamental plant with highly medicinal val-ue, are being seriously threatened due to over collection , and are becoming endangered species .For a success cultivation and breeding of Cypripedium, mycorrhizal fungus is playing a very important role in this process .In this test , mycorrhizal fungus extracted from C.yunnanense and C.guttatum which distributed in northwest Yunnan , were studied through method of rDNA ITS ( internal transcribed spacer ) sequences analysis , and MEGA software .The results showed that there was significant relation between the mycorrhizal fungus of C.yunnanense and that of C.guttatum, in terms of specificity .In another word , mycorrhizal fungus has a obvious tendency in symbiosis with specific Cypripedium.The sequences of mycorrhizal fungus were uploaded to NCBI , and the access numbers were lcl51879, lcl25153, lcl48033, lcl38377, lcl14203, lcl24557, lcl52287 and lcl46937.