生物学杂志
生物學雜誌
생물학잡지
JOURNAL OF BIOLOGY
2014年
3期
104-107
,共4页
莫日根%邢万金%王志钢%阿拉坦高勒%哈斯阿古拉%苑琳%侯鑫%苏慧敏%张彦桃%王郑%范丽菲
莫日根%邢萬金%王誌鋼%阿拉坦高勒%哈斯阿古拉%苑琳%侯鑫%囌慧敏%張彥桃%王鄭%範麗菲
막일근%형만금%왕지강%아랍탄고륵%합사아고랍%원림%후흠%소혜민%장언도%왕정%범려비
实验教学%生物技术%基因工程
實驗教學%生物技術%基因工程
실험교학%생물기술%기인공정
experimental teaching%biotechnology%genetic engineering
为了培养本科生的分子生物学实验设计与操作技能，在实践教学中实现培养本科创新人才的目标，以用大肠杆菌发酵生产重组细菌碱性磷酸酶为案例，通过碱性磷酸酶基因的克隆、原核表达、发酵生产、提取纯化以及酶活性检测等系列实验，把本科的基因工程、发酵工程和生物化学3门综合性独立实验课程有机地组合成一个内容相关联的超大型综合性生物技术大实验，进一步凸显了生物技术中以基因工程技术为核心的上游核酸操作、中游发酵生产和下游蛋白分离纯化三大技术模块的有机联系，大大地提高了本科实验教学的综合性和研究性，提升了实践教学水平，取得了良好的教学效果。
為瞭培養本科生的分子生物學實驗設計與操作技能，在實踐教學中實現培養本科創新人纔的目標，以用大腸桿菌髮酵生產重組細菌堿性燐痠酶為案例，通過堿性燐痠酶基因的剋隆、原覈錶達、髮酵生產、提取純化以及酶活性檢測等繫列實驗，把本科的基因工程、髮酵工程和生物化學3門綜閤性獨立實驗課程有機地組閤成一箇內容相關聯的超大型綜閤性生物技術大實驗，進一步凸顯瞭生物技術中以基因工程技術為覈心的上遊覈痠操作、中遊髮酵生產和下遊蛋白分離純化三大技術模塊的有機聯繫，大大地提高瞭本科實驗教學的綜閤性和研究性，提升瞭實踐教學水平，取得瞭良好的教學效果。
위료배양본과생적분자생물학실험설계여조작기능，재실천교학중실현배양본과창신인재적목표，이용대장간균발효생산중조세균감성린산매위안례，통과감성린산매기인적극륭、원핵표체、발효생산、제취순화이급매활성검측등계렬실험，파본과적기인공정、발효공정화생물화학3문종합성독립실험과정유궤지조합성일개내용상관련적초대형종합성생물기술대실험，진일보철현료생물기술중이기인공정기술위핵심적상유핵산조작、중유발효생산화하유단백분리순화삼대기술모괴적유궤련계，대대지제고료본과실험교학적종합성화연구성，제승료실천교학수평，취득료량호적교학효과。
In order to introduce a complete technique of biotechnology into an undergraduate experimental course , the bacterial alkaline phosphatase gene was chosen as an example to be cloned and over-expressed in Escherichia coli cells.The alkaline phosphatase was ex-pected to be produced in large scale through fermentation , and the subsequent product could be purified and analyzed by biochemical methods .The design organically integrated our original three individual experimental courses -gene engineering , microbial fermentation and biochemistry as a super comprehensive course of biotechnology .The course included three tightly-related, but also relatively sepa-rated substantial domains-an up-stream module for alkaline phosphatase gene manipulation , a middle-stream module for microbial fer-mentation of alkaline phosphatase , and a down-stream module for biochemistry of alkaline phosphatase .Here, the paper briefly re-viewed the break-through in biotechnology and described the contents and practical arrangement of each module in a proper semester , establishing the necessity to set such a comprehensive course in training creative talents of biotechnology for the future .
