CAJ | 학술논문

目前，国内普遍采用亮菌固体发酵工艺生产亮菌制剂。采用小型发酵罐液体深层发酵和液体静置发酵对亮菌发酵工艺进行优化研究。液体深层发酵采用28℃，200 r/min，通气量1∶1 v/v· m，培养7 d，亮菌干重为16.55 g/L，其中菌丝体中多糖含量为5.42％，蛋白含量为1.75％；液体静置发酵采用500 mL三角瓶，28℃，150 r/min ，摇瓶3 d后静置发酵14 d，亮菌干重可达10.69 g/L，发酵液中多糖含量为1.016 g/L，蛋白含量为0.320 g/L。对液体静置发酵进一步研究发现其发酵液中亮菌甲素含量可达3.118 mg/L。由此可见，两种发酵方式在保证生物量和活性成分的前提下，缩短了发酵周期，均优于传统的固体发酵工艺，值得工业生产借鉴。
목전，국내보편채용량균고체발효공예생산량균제제。채용소형발효관액체심층발효화액체정치발효대량균발효공예진행우화연구。액체심층발효채용28℃，200 r/min，통기량1∶1 v/v· m，배양7 d，량균간중위16.55 g/L，기중균사체중다당함량위5.42％，단백함량위1.75％；액체정치발효채용500 mL삼각병，28℃，150 r/min ，요병3 d후정치발효14 d，량균간중가체10.69 g/L，발효액중다당함량위1.016 g/L，단백함량위0.320 g/L。대액체정치발효진일보연구발현기발효액중량균갑소함량가체3.118 mg/L。유차가견，량충발효방식재보증생물량화활성성분적전제하，축단료발효주기，균우우전통적고체발효공예，치득공업생산차감。
At present , preparation of Armillariella tabescens was generally carried on with solid state fermentation .The optimization of fermentation technology of Armillariella tabescens with taking the deep liquid fermentation of fermentor and stationary liquid fermentation methods was made .The deep liquid fermentation was studied under the condition of 28℃, 200 r/min, ventilation of 1∶1 v/v· m.Af-ter culturation for 7 days, the biomass of Armillariella tabescens in dry weight was 16.55 g/L, and the mycelia contained polysaccharide 5.42%, protein 1.75%;the stationary liquid fermentation was taken firstly by using 500 mL conical flask to shake for 3 days at 28℃, 150 r/min, and then to stationary culture for 14 days, the biomass of Armillariella tabescens in dry weight could reach to 10.69 g/L, and the fermentation broth included polysaccharide 1.016 g/L, protein 0.320 g/L.