CAJ | 학술논문

雌雄配子间的结合与融合是哺乳动物授精成功的关键步骤，哺乳动物富含半胱氨酸的分泌蛋白CRISPs家族是一个进化上高度保守的蛋白家族，参与了精卵结合与融合过程，并在其中扮演了多种角色。目前从雄性小鼠生殖道中分离出4个CRISPs家族成员：附睾的CRISP1、睾丸的CRISP2、分布广泛的CRISP3以及与人CRISP1同源的CRISP4，对CRISPs家族蛋白成员的晶体结构分析揭示出CRISP蛋白含有两个功能域，一个是位于N末端的结构保守的CAP结构域，另一个是位于C末端的CRISP蛋白家族特有的CRISP功能域。 CAP功能域中含有CAP基序， CRISP功能域由一个短的铰链区和一个离子通道调节区组成，并通过铰链区与CAP结构域相连接。简要回顾了各种CRISP蛋白的发现和特性鉴别过程，希望能从CRISPs的角度对哺乳动物精卵识别、结合与融合的分子机制有更好的了解。
자웅배자간적결합여융합시포유동물수정성공적관건보취，포유동물부함반광안산적분비단백CRISPs가족시일개진화상고도보수적단백가족，삼여료정란결합여융합과정，병재기중분연료다충각색。목전종웅성소서생식도중분리출4개CRISPs가족성원：부고적CRISP1、고환적CRISP2、분포엄범적CRISP3이급여인CRISP1동원적CRISP4，대CRISPs가족단백성원적정체결구분석게시출CRISP단백함유량개공능역，일개시위우N말단적결구보수적CAP결구역，령일개시위우C말단적CRISP단백가족특유적CRISP공능역。 CAP공능역중함유CAP기서， CRISP공능역유일개단적교련구화일개리자통도조절구조성，병통과교련구여CAP결구역상련접。간요회고료각충CRISP단백적발현화특성감별과정，희망능종CRISPs적각도대포유동물정란식별、결합여융합적분자궤제유경호적료해。
Gamete binding and fusion are essential to fertilization in mammals .A family of cysteine-rich secretory proteins ( CRISPs) in mammals is a highly conserved protein family in evolution , it is involved in the progresses to play multiple roles in mammalian sperm-egg fusion.To date, four members of this family were isolated mainly from the male mouse reproductive tract , epididymal CRISP1, testicular CRISP2, widespread CRISP3 and CRISP4, and a human CRISP1 ortholog.Crystallographic analysis of a CRISP family member revealed that CRISPs contain two domains , a structurally conserved N-terminal CAP ( cysteine-rich secretory proteins (CRISPS), antigen 5 (Ag5), and pathogenesis-related 1 (Pr-1)) domain containing CAP motifs, and a C-terminal cysteine-rich domain ( CRD) only presenting in CRISPs .The CRD domain is comprised of a short Hinge region and an ICR ( ion channel regulator ) region and is connected to the CAP domain by the Hinge region .This article presented a detail review of identification and characteriza-tion of CRISPs for better understanding of the molecular mechanisms of mammalian sperm -egg fusion in view of CRISPs .