CAJ | 학술논문

以马铃薯抗青枯病二倍体材料ED13和CE171、炸片颜色好的二倍体材料HS66以及优良性状双单体材料DH401和DH405为供体材料，对马铃薯叶肉原生质体培养进行研究。叶片悬浮黑暗预处理和试管苗黑暗预处理两种预处理方式对原生质体活力无显著影响。以0.5 mol/L甘露醇为渗透调节剂，25℃酶解12 h条件下，适宜CE171和DH401纤维酶浓度略高于ED13、HS66和DH405，分别为0.3％和0.4％。 ED13和DH401原生质体在VKM液体培养基中培养3～4周，经愈伤组织生长培养基培养2周，转至芽诱导培养基培养，2～3个月后形成具根茎叶的完整植株。 HS66和CE171原生质体培养6～8周也能形3～4 mm愈伤组织，但没有分化出芽；DH405的原生质体不分裂。
이마령서항청고병이배체재료ED13화CE171、작편안색호적이배체재료HS66이급우량성상쌍단체재료DH401화DH405위공체재료，대마령서협육원생질체배양진행연구。협편현부흑암예처리화시관묘흑암예처리량충예처리방식대원생질체활력무현저영향。이0.5 mol/L감로순위삼투조절제，25℃매해12 h조건하，괄의CE171화DH401섬유매농도략고우ED13、HS66화DH405，분별위0.3％화0.4％。 ED13화DH401원생질체재VKM액체배양기중배양3～4주，경유상조직생장배양기배양2주，전지아유도배양기배양，2～3개월후형성구근경협적완정식주。 HS66화CE171원생질체배양6～8주야능형3～4 mm유상조직，단몰유분화출아；DH405적원생질체불분렬。
The studies on protoplast culture for diploid potato with resistant to bacterial wilt or with good chip color ( ED13 , CE171 , and HS66) and ones with good agricultural traits (DH401 and DH405) showed that there were no significant difference of protoplast yield and viability between leaves in suspension medium in dark and complete plantlets in dark for pretreatment .When 0.5 mol/L mannitol was used as an osmosis regulator , the suitable cellulose concentration (0.4%) for protoplast isolation of CE171 and DH401 was higher than those of ED13, HS66 and DH401(0.3%) at 25℃ for 12 h.The protoplast of ED13, DH401, CE171, and HS66 could be induced into callus in VKM liquid medium .The protoplast of ED13 and DH401 could regenerate into complete plantlets , but protoplast of DH405 had no division capability .