CAJ | 학술논문

为探讨铁皮石斛(Dendrobium officinale)培养基中添加物的作用，在1/2MS培养基中加入椰肉、甘蔗渣、香蕉皮和麦麸等4种添加物，研究不同浓度添加物和培养时间对原球茎生长和多糖含量的影响。结果表明，4种添加物对铁皮石斛原球茎的增殖、分化和多糖含量均有一定影响，其中添加15.0 g L-1甘蔗渣，培养60 d能明显促进铁皮石斛原球茎的增殖与分化(146.1%)；而添加20.0 g L-1甘蔗渣，培养40 d能显著提高铁皮石斛原球茎多糖含量(50.4%)。这说明甘蔗渣是培养铁皮石斛原球茎的适宜添加物，既能促进铁皮石斛原球茎的生长发育，还能降低生产成本。
위탐토철피석곡(Dendrobium officinale)배양기중첨가물적작용，재1/2MS배양기중가입야육、감자사、향초피화맥부등4충첨가물，연구불동농도첨가물화배양시간대원구경생장화다당함량적영향。결과표명，4충첨가물대철피석곡원구경적증식、분화화다당함량균유일정영향，기중첨가15.0 g L-1감자사，배양60 d능명현촉진철피석곡원구경적증식여분화(146.1%)；이첨가20.0 g L-1감자사，배양40 d능현저제고철피석곡원구경다당함량(50.4%)。저설명감자사시배양철피석곡원구경적괄의첨가물，기능촉진철피석곡원구경적생장발육，환능강저생산성본。
In order to understand the function of additive in medium of Dendrobium officinale, four additives, including coconut meat, sugarcane bagasse, banana peel and wheat bran, were supplied in 1/2MS medium, respectively. The effects of additives with different concentrates and culture time on growth and polysaccharide content of D. officinale PLBs were studied. The results showed that the PLBs proliferation, differentiation and the polysaccharide content were inlfuenced by four additives. The proliferation rate of PBLs reached up to 146.1%supplemented with 15.0 g L-1 sugarcane bagasse and cultured for 60 d. The polysaccharide content of PLBs was increased for 50.4%supplemented with 20.0 g L-1 sugarcane bagasse and cultured for 40 days. So it was suggested that sugarcane bagasse was suitable additive for in vitro D. officinale PLBs owing to promote the growth of D. officinale PLBs and reduce cost of production.