医学分子生物学杂志
醫學分子生物學雜誌
의학분자생물학잡지
FOREIGN MEDICAL SCIENCES
2014年
3期
175-178
,共4页
C/EBPβ%TGF-β/Smad3通路%星型胶质细胞%交通性脑积水
C/EBPβ%TGF-β/Smad3通路%星型膠質細胞%交通性腦積水
C/EBPβ%TGF-β/Smad3통로%성형효질세포%교통성뇌적수
C/EBPβ%TGF-β/Smad3 pathway%astrocytes%communicating hydrocephalus
目的：探讨C/EBPβ在星型胶质细胞中对TGF-β/Smad3通路的调控及分子机制。方法采用实时荧光定量PCR和Western印迹的方法分别测定上调星形胶质细胞中C/EBPβ和Smad3的表达对TGF-β及Smad3的影响。并通过上调和沉默细胞中C/EBPβ的表达测定其对细胞增殖及凋亡的影响。结果上调星形胶质细胞中C/EBPβ的表达显著增强了TGF-β及Smad3的表达水平，而上调Smad3的表达则降低了TGF-β的表达；上调星形胶质细胞中C/EBPβ的表达与对照组相比，细胞增殖率提高[（73．26±4．57）％ VS （138．29±3．36）％]，凋亡率降低[（24．69±2．06）％VS （6．32±1．63）％]；沉默细胞中C/EBPβ的表达与对照组相比，细胞增殖率降低[（73．26±4．57）％VS （97．54±3．72）％]，凋亡率升高[（24．69±2．06）％VS （14．76±1．43）％]，差异具有统计学意义（P＜0．01）。结论 C/EBPβ可通过诱导TGF-β的表达而激活星型胶质细胞中的TGF-β/Smad3信号转导通路，并促进星形胶质细胞增殖。
目的：探討C/EBPβ在星型膠質細胞中對TGF-β/Smad3通路的調控及分子機製。方法採用實時熒光定量PCR和Western印跡的方法分彆測定上調星形膠質細胞中C/EBPβ和Smad3的錶達對TGF-β及Smad3的影響。併通過上調和沉默細胞中C/EBPβ的錶達測定其對細胞增殖及凋亡的影響。結果上調星形膠質細胞中C/EBPβ的錶達顯著增彊瞭TGF-β及Smad3的錶達水平，而上調Smad3的錶達則降低瞭TGF-β的錶達；上調星形膠質細胞中C/EBPβ的錶達與對照組相比，細胞增殖率提高[（73．26±4．57）％ VS （138．29±3．36）％]，凋亡率降低[（24．69±2．06）％VS （6．32±1．63）％]；沉默細胞中C/EBPβ的錶達與對照組相比，細胞增殖率降低[（73．26±4．57）％VS （97．54±3．72）％]，凋亡率升高[（24．69±2．06）％VS （14．76±1．43）％]，差異具有統計學意義（P＜0．01）。結論 C/EBPβ可通過誘導TGF-β的錶達而激活星型膠質細胞中的TGF-β/Smad3信號轉導通路，併促進星形膠質細胞增殖。
목적：탐토C/EBPβ재성형효질세포중대TGF-β/Smad3통로적조공급분자궤제。방법채용실시형광정량PCR화Western인적적방법분별측정상조성형효질세포중C/EBPβ화Smad3적표체대TGF-β급Smad3적영향。병통과상조화침묵세포중C/EBPβ적표체측정기대세포증식급조망적영향。결과상조성형효질세포중C/EBPβ적표체현저증강료TGF-β급Smad3적표체수평，이상조Smad3적표체칙강저료TGF-β적표체；상조성형효질세포중C/EBPβ적표체여대조조상비，세포증식솔제고[（73．26±4．57）％ VS （138．29±3．36）％]，조망솔강저[（24．69±2．06）％VS （6．32±1．63）％]；침묵세포중C/EBPβ적표체여대조조상비，세포증식솔강저[（73．26±4．57）％VS （97．54±3．72）％]，조망솔승고[（24．69±2．06）％VS （14．76±1．43）％]，차이구유통계학의의（P＜0．01）。결론 C/EBPβ가통과유도TGF-β적표체이격활성형효질세포중적TGF-β/Smad3신호전도통로，병촉진성형효질세포증식。
Objective To examine the regulatory effect of C/EBPβon the TGF-β/Smad3 path-way in astrocytes and the molecular mechanism .Methods Real-time PCR and Western blotting were used to examine the effect of up-regulated expressions of C/EBPβand Smad3 on TGF-βin as-trocytes.The proliferation rate and the apoptosis rate of astrocytes were detected after up-regulation or silencing of the C/EBPβgene.Results The expression levels of TGF-βand Smad3 were obviously increased in astrocytes overexpressing C/EBPβ, while the expression level of TGF-βwas decreased in astrocytes overexpressing Smad 3.The proliferation rate was significantly elevated and the apoptosis rate declined in astrocytes with C/EBPβgene up-regulated [ ( 73.26 ±4.57 )%vs. ( 138.29 ± 3.36)%] for proliferation rate; [ (24.69 ±2.06)%vs.(6.32 ±1.63)%] for apoptosis rate, P<0.01) .In astrocytes with silencing of C/EBPβ, the proliferation rate [ (73.26 ±4.57)%vs. (97.54 ±3.72)%] was significantly declined and the apoptosis rate elevated [ (24.69 ±2.06)%vs.(14.76 ±1.43)%, P<0.01] .Conclusion C/EBPβcan activate the TGF-β/Smad3 path-way in astrocytes and promote the proliferation of astrocytes by inducing the expression of TGF-β.
