CAJ | 학술논문

目的探讨PTEN基因在乳腺癌细胞中的表达及对细胞生物学行为的影响.方法选择PTEN正常表达的细胞株MDA-MB-231(M231),使用ShRNA方法沉默PTEN的表达,构建PTEN低表达的M231细胞株(M231-3001)及对照组细胞(M231-scr),用RT-PCR、Western-blot方法分析目的基因的表达抑制,通过CCK-8法、细胞划痕实验和Transwell细胞迁移实验等方法检测PTEN基因沉默后乳腺癌细胞株M231增殖、迁移及侵袭能力的改变.结果与对照组M231-scr相比,M231-3001细胞株的PTENmRNA和蛋白的表达明显降低(P＜0.01),M231-3001细胞株相对于M231-scr细胞株增生、繁殖力增强,同时迁移和侵袭能力增加(均P ＜0.05).结论 PTEN基因具有抑癌作用,其表达降低可促进癌细胞增生、迁移和侵袭.
목적 탐토PTEN기인재유선암세포중적표체급대세포생물학행위적영향.방법 선택PTEN정상표체적세포주MDA-MB-231(M231),사용ShRNA방법침묵PTEN적표체,구건PTEN저표체적M231세포주(M231-3001)급대조조세포(M231-scr),용RT-PCR、Western-blot방법분석목적기인적표체억제,통과CCK-8법、세포화흔실험화Transwell세포천이실험등방법검측PTEN기인침묵후유선암세포주M231증식、천이급침습능력적개변.결과 여대조조M231-scr상비,M231-3001세포주적PTENmRNA화단백적표체명현강저(P＜0.01),M231-3001세포주상대우M231-scr세포주증생、번식력증강,동시천이화침습능력증가(균P ＜0.05).결론 PTEN기인구유억암작용,기표체강저가촉진암세포증생、천이화침습.
Objective To investigate expression of the phosphatase and tensin homolog (PTEN) gene in breast cancer cell line and its effect on biologic characteristics.Methods The normal PTEN expression cell line MDA-MB-231 (M231) was used in this study.PTEN-shRNA plasmid was transected into M231 breast cancer cells to knock down the expression of PTEN.The changes of PTEN expression,proliferation,invasion and metastasis of PTEN knocked down cell were tested by RT-PCR,Western blot,CCK-8,scratch and Transwell.Results PTEN-shRNA was successfully transected into M231 cells.PTEN mRNA and protein expression was efficiently inhibited in M231-3001 cell lines than that in control group M231-scr(P ＜ 0.01),M231-3001 cell lines showed a greater capability of colony formation,migration and invasion than that in control group M231-scr (all P ＜ 0.05).Conclusions PTEN,as a suppression gene,its low expression can promote the proliferation,migration and invasion of breast cancer cells.