CAJ | 학술논문

目的：为了筛选出适合用于组织工程皮肤的膜支架材料，采用不同来源的纤维素（木、棉、竹），与壳聚糖以及海藻酸钠等多糖为原料制备了多种复合膜，并表征了其理化性能。方法分别配制木、棉、竹纤维素溶液，海藻酸钠溶液和壳聚糖溶液，分别采用木、棉、竹3种纤维素溶液作为基底膜，然后依次流延一层壳聚糖膜和一层海藻酸钠膜，从而制得3种不同来源的纤维素-壳聚糖-海藻酸盐复合膜。应用荧光倒置显微镜观察3种复合膜的微观形态结构。将复合膜浸泡在生理盐水中，观察复合膜的形态变化来判定复合膜的盐水耐受性。运用接触角分析仪观察并测定不同复合膜与水的接触角的大小及其随时间的变化。结果在荧光倒置显微镜下观察到木纤维素复合膜中的纤维粗而短，棉纤维素复合膜中的纤维粗而长，竹纤维素复合膜中的纤维细而长且交错排列形成网状膜结构。通过将复合膜浸泡在生理盐水中观察到木纤维素复合膜和棉纤维素复合膜在第15天时出现了裂痕，而竹纤维素复合膜则没有。不同复合膜与水的接触角大小及其随时间的变化表明竹纤维素复合膜的亲水性较好。结论本研究制备了一种以壳聚糖、海藻酸钠、纤维素为原材料的复合膜组织工程皮肤。通过比较3种复合膜的理化性能，结果表明竹纤维基复合膜中的纤维之间容易形成互穿网状结构，所以结合较紧密，盐水耐受性好。
목적：위료사선출괄합용우조직공정피부적막지가재료，채용불동래원적섬유소（목、면、죽），여각취당이급해조산납등다당위원료제비료다충복합막，병표정료기이화성능。방법분별배제목、면、죽섬유소용액，해조산납용액화각취당용액，분별채용목、면、죽3충섬유소용액작위기저막，연후의차류연일층각취당막화일층해조산납막，종이제득3충불동래원적섬유소-각취당-해조산염복합막。응용형광도치현미경관찰3충복합막적미관형태결구。장복합막침포재생리염수중，관찰복합막적형태변화래판정복합막적염수내수성。운용접촉각분석의관찰병측정불동복합막여수적접촉각적대소급기수시간적변화。결과재형광도치현미경하관찰도목섬유소복합막중적섬유조이단，면섬유소복합막중적섬유조이장，죽섬유소복합막중적섬유세이장차교착배렬형성망상막결구。통과장복합막침포재생리염수중관찰도목섬유소복합막화면섬유소복합막재제15천시출현료렬흔，이죽섬유소복합막칙몰유。불동복합막여수적접촉각대소급기수시간적변화표명죽섬유소복합막적친수성교호。결론본연구제비료일충이각취당、해조산납、섬유소위원재료적복합막조직공정피부。통과비교3충복합막적이화성능，결과표명죽섬유기복합막중적섬유지간용역형성호천망상결구，소이결합교긴밀，염수내수성호。
Objective To prepare a variety of complex films with alginate , chitosan , and cel-lulose of different origins in order to identify the optimal film for tissue engineering skin .Methods The sodium alginate , chitosan and cellulose ( cotton, wood and bamboo ) solutions were prepared re-spectively.The complex films were prepared by using the cellulose (cotton, wood and bamboo) film as the basement , and then casting one layer of sodium alginate and another layer of chitosan on the basement in turn.Fluorescence inverted microscopy was used to observe the microstructures of the three kinds of complex films .The complex films were immersed in physiological saline and the mor-phology of the complex films was observed to examine the salt tolerance property .Contact angle ana-lyzer was used to observe contact angles and their changes along with time in water to test their hy -drophilic property.Results The microstructures of the three kinds of complex films under fluores-cence inverted microscope illustrated that the interpenetrating network structure was firmed in the in the bamboo cellulose-based complex film with long and slim fiber , whereas cotton cellulose-based complex films had long and thick fiber , and wood cellulose-based complex film contained short and thick fibers.After complex films were immersed in physiological saline , it was observed that the bamboo cellulose-based film was still smooth and had no cracks within 15 days, whereas the wood cellulose-based film and cotton cellulose-based film developed cracks .The changes of contact angle with water in different complex films indicated that the hydrophilic property of bamboo cellulose-based film was the best.Conclusion A complex film was prepared by using chitosan , sodium algi-nate, and cellulose as raw materials.The bamboo cellulose-based complex film had good salt toler-ance and hydrophilicity due to its interpenetrating network structure and it can be the optimal com -plex film for tissue engineering skin .