医学分子生物学杂志
醫學分子生物學雜誌
의학분자생물학잡지
FOREIGN MEDICAL SCIENCES
2014年
3期
150-154
,共5页
希尔扎提江?苏来曼%阿丽叶古丽?艾皮热%热孜宛古丽?约麦尔%吾尼且木?吐拉克%刘开江%Abulizi Abudula
希爾扎提江?囌來曼%阿麗葉古麗?艾皮熱%熱孜宛古麗?約麥爾%吾尼且木?吐拉剋%劉開江%Abulizi Abudula
희이찰제강?소래만%아려협고려?애피열%열자완고려?약맥이%오니차목?토랍극%류개강%Abulizi Abudula
宫颈鳞状细胞癌%维吾尔族妇女%清道夫受体A类5型
宮頸鱗狀細胞癌%維吾爾族婦女%清道伕受體A類5型
궁경린상세포암%유오이족부녀%청도부수체A류5형
cervical squamous cell carcinoma%Uyghur women%scavenger receptor class A member 5 ( putative )
目的：在前期研究中，发现宫颈鳞癌组织中清道夫受体A类5型（ scavenger receptor class A member 5 putative， SCARA5）基因表达下调。本研究利用宫颈癌及癌前病变组织标本对SCARA5基因的mR-NA和蛋白质表达水平进行定量分析，探讨宫颈癌发生与该基因表达调控的关系及临床意义。方法收集维吾尔族妇女宫颈鳞癌（ cervical squamous cell carcinoma ， CSCC ）、宫颈内上皮瘤变（ cervical intraepithelial neoplasia， CINⅡ～Ⅲ）及宫颈炎患者的新鲜组织标本72例及石蜡包埋组织切片82例，采用定量RT-PCR和免疫组织化学方法，对SCARA5基因转录（ mRNA）和蛋白质表达水平进行鉴定。结果随着宫颈炎发展到CIN和CSCC， SCARA5基因的转录（ mRNA）表达水平梯度性下调， CSCC与CIN或宫颈炎之间差异显著（P＜0．05），但是CIN与宫颈炎的差异无统计学意义。宫颈炎组织内SCARA5蛋白表达呈强阳性，在CINⅡ～Ⅲ中的缺失率增加，在宫颈癌阶段，其部分或完全缺失率最高，与宫颈炎存在显著差异（ P＜0．05）。结论宫颈癌发生与SCARA5转录表达下调或蛋白质表达缺失存在密切关系，可能成为肿瘤诊断和预后的分子标志物。
目的：在前期研究中，髮現宮頸鱗癌組織中清道伕受體A類5型（ scavenger receptor class A member 5 putative， SCARA5）基因錶達下調。本研究利用宮頸癌及癌前病變組織標本對SCARA5基因的mR-NA和蛋白質錶達水平進行定量分析，探討宮頸癌髮生與該基因錶達調控的關繫及臨床意義。方法收集維吾爾族婦女宮頸鱗癌（ cervical squamous cell carcinoma ， CSCC ）、宮頸內上皮瘤變（ cervical intraepithelial neoplasia， CINⅡ～Ⅲ）及宮頸炎患者的新鮮組織標本72例及石蠟包埋組織切片82例，採用定量RT-PCR和免疫組織化學方法，對SCARA5基因轉錄（ mRNA）和蛋白質錶達水平進行鑒定。結果隨著宮頸炎髮展到CIN和CSCC， SCARA5基因的轉錄（ mRNA）錶達水平梯度性下調， CSCC與CIN或宮頸炎之間差異顯著（P＜0．05），但是CIN與宮頸炎的差異無統計學意義。宮頸炎組織內SCARA5蛋白錶達呈彊暘性，在CINⅡ～Ⅲ中的缺失率增加，在宮頸癌階段，其部分或完全缺失率最高，與宮頸炎存在顯著差異（ P＜0．05）。結論宮頸癌髮生與SCARA5轉錄錶達下調或蛋白質錶達缺失存在密切關繫，可能成為腫瘤診斷和預後的分子標誌物。
목적：재전기연구중，발현궁경린암조직중청도부수체A류5형（ scavenger receptor class A member 5 putative， SCARA5）기인표체하조。본연구이용궁경암급암전병변조직표본대SCARA5기인적mR-NA화단백질표체수평진행정량분석，탐토궁경암발생여해기인표체조공적관계급림상의의。방법수집유오이족부녀궁경린암（ cervical squamous cell carcinoma ， CSCC ）、궁경내상피류변（ cervical intraepithelial neoplasia， CINⅡ～Ⅲ）급궁경염환자적신선조직표본72례급석사포매조직절편82례，채용정량RT-PCR화면역조직화학방법，대SCARA5기인전록（ mRNA）화단백질표체수평진행감정。결과수착궁경염발전도CIN화CSCC， SCARA5기인적전록（ mRNA）표체수평제도성하조， CSCC여CIN혹궁경염지간차이현저（P＜0．05），단시CIN여궁경염적차이무통계학의의。궁경염조직내SCARA5단백표체정강양성，재CINⅡ～Ⅲ중적결실솔증가，재궁경암계단，기부분혹완전결실솔최고，여궁경염존재현저차이（ P＜0．05）。결론궁경암발생여SCARA5전록표체하조혹단백질표체결실존재밀절관계，가능성위종류진단화예후적분자표지물。
Objective Previous studies have shown that the expression of scavenger receptor class A, member 5 (putative) (SCARA5) was down-regulated in cervical squamous cell carcinoma (CSCC) .The aim of this study was to investigate the association of regulation of SCARA 5 expres-sion with cervical carcinogenesis by quantitatively analyzing the mRNA and protein expression level of this gene in cervical carcinoma and its precursor lesions .Methods A total of 72 fresh tissue sam-ples and 82 paraffin-embedded tissue slides were collected from Uyghur women with CSCC , cervical intraepithelial neoplasia (CIN) Ⅱ-Ⅲor cervicitis, or healthy Uyghur individuals.The mRNA and protein expression of SCARA 5 gene was determined by quantitative RT-PCR and immunohistochem-istry, respectively.Result The mRNA expression level of SCARA 5 was gradually down-regulated in cervicitis, CIN and CSCC in order , which reached statistically significant difference between CSCC and CIN or cervicitis ( P<0.05 ) .But no difference was found in the mRNA expression of SCARA 5 between CIN and cervicitis.Cervicitis tissues were strong-positive for SCARA5 protein, loss of SCARA5 expression increased in CINⅡ-Ⅲ, and the rate of partial or total loss of SCARA 5 expres-sion was highest in CSCC , which was significantly different from that in cervicitis ( P <0.05 ) .Conclusion The down-regulation of SCARA5 transcription or loss of protein expression is closely associated with cervical cancer development , and SCARA5 may become a molecular marker for the diagnosis and prognosis of CSCC .
