医学分子生物学杂志
醫學分子生物學雜誌
의학분자생물학잡지
FOREIGN MEDICAL SCIENCES
2014年
3期
144-149
,共6页
人类细胞周期素D%CCND1%CCND2%CCND3%分子进化
人類細胞週期素D%CCND1%CCND2%CCND3%分子進化
인류세포주기소D%CCND1%CCND2%CCND3%분자진화
cyclin D%CCND1%CCND2%CCND3%molecular evolution
目的：对细胞周期素（cyclin D）家族成员进行分子生物学进化及功能预测，为临床上检测骨质疏松提供更多分子生物学信息。方法对CCND基因家族成员包括CCND1、 CCND2和CCND3序列进行分子进化分析，并在所有科属范围和人型总科范围内，计算了同源CCNDs之间的同义和非同义突变频率。结果 CCND1和CCND2具有较高的序列相同性，而CCND3的非同义突变率较高，尤其在人型总科各属形成以后，平均每年的同义和非同义突变频率都高于CCND1和CCND2。结论 CCND2与CCND1序列相同程度高，而CCND3在序列和进化速率上都显著不同，所以CCND2可以作为骨密度关联研究的候选基因。
目的：對細胞週期素（cyclin D）傢族成員進行分子生物學進化及功能預測，為臨床上檢測骨質疏鬆提供更多分子生物學信息。方法對CCND基因傢族成員包括CCND1、 CCND2和CCND3序列進行分子進化分析，併在所有科屬範圍和人型總科範圍內，計算瞭同源CCNDs之間的同義和非同義突變頻率。結果 CCND1和CCND2具有較高的序列相同性，而CCND3的非同義突變率較高，尤其在人型總科各屬形成以後，平均每年的同義和非同義突變頻率都高于CCND1和CCND2。結論 CCND2與CCND1序列相同程度高，而CCND3在序列和進化速率上都顯著不同，所以CCND2可以作為骨密度關聯研究的候選基因。
목적：대세포주기소（cyclin D）가족성원진행분자생물학진화급공능예측，위림상상검측골질소송제공경다분자생물학신식。방법대CCND기인가족성원포괄CCND1、 CCND2화CCND3서렬진행분자진화분석，병재소유과속범위화인형총과범위내，계산료동원CCNDs지간적동의화비동의돌변빈솔。결과 CCND1화CCND2구유교고적서렬상동성，이CCND3적비동의돌변솔교고，우기재인형총과각속형성이후，평균매년적동의화비동의돌변빈솔도고우CCND1화CCND2。결론 CCND2여CCND1서렬상동정도고，이CCND3재서렬화진화속솔상도현저불동，소이CCND2가이작위골밀도관련연구적후선기인。
Objective To provide more molecular biology information for clinical detection of osteoporosis , we have studied the molecular evolution and functional prediction of Cyclin D gene family.Method: Phylogenetic analysis of Cyclin D , including Cyclin D1, Cyclin D2 and Cyclin D3, has been carried out.Meanwhile, synonymous mutation frequency and nonsynonymous muta-tion frequency among homologous CCNDs from all the studied species were calculated .Results The results showed that CCND 1 and CCND2 exhibited higher identity with each other , and high frequen-cy of nonsynonymous mutation occurred in CCND 3.In particular , after the divergence of homi-noidea , annual average synonymous and nonsynonymous mutation frequencies were higher in CC -ND3 than in CCND1 and CCND2.Conclusion Our results showed that CCND 2 was much identical to CCND1 and CCND3 was significantly different from other two CCNDs based on both sequence i-dentity and molecular evolution rate .And CCND2 can be used as a candidate gene of osteoporosis .
