蚌埠医学院学报
蚌埠醫學院學報
방부의학원학보
ACTA ACADEMIAE MEDICINAE BENGBU
2014年
5期
564-567
,共4页
范高福%童旭辉%董淑英%蒋国君%陶亮
範高福%童旭輝%董淑英%蔣國君%陶亮
범고복%동욱휘%동숙영%장국군%도량
鞣花酸%连接蛋白%缝隙连接
鞣花痠%連接蛋白%縫隙連接
유화산%련접단백%봉극련접
ellagic acid%connexin%gap junction
目的：观察鞣花酸( EA)对转染并稳定表达Cx32的HeLa细胞中缝隙连接( GJ)通讯功能的影响。方法：细胞接种荧光示踪法观察EA对GJ功能的影响；细胞免疫荧光法检测EA对细胞膜上Cx32表达的影响。结果：EA 4、8、16μmol/L分别使细胞荧光渗透率显著增强6.04%、12.50%和20.33%(P<0.01)，使细胞膜Cx32的荧光强度显著增强23.66%、46.09%和92.18%(P<0.01)。结论：EA可通过增强胞膜Cx32的表达，增强细胞GJ通讯功能。
目的：觀察鞣花痠( EA)對轉染併穩定錶達Cx32的HeLa細胞中縫隙連接( GJ)通訊功能的影響。方法：細胞接種熒光示蹤法觀察EA對GJ功能的影響；細胞免疫熒光法檢測EA對細胞膜上Cx32錶達的影響。結果：EA 4、8、16μmol/L分彆使細胞熒光滲透率顯著增彊6.04%、12.50%和20.33%(P<0.01)，使細胞膜Cx32的熒光彊度顯著增彊23.66%、46.09%和92.18%(P<0.01)。結論：EA可通過增彊胞膜Cx32的錶達，增彊細胞GJ通訊功能。
목적：관찰유화산( EA)대전염병은정표체Cx32적HeLa세포중봉극련접( GJ)통신공능적영향。방법：세포접충형광시종법관찰EA대GJ공능적영향；세포면역형광법검측EA대세포막상Cx32표체적영향。결과：EA 4、8、16μmol/L분별사세포형광삼투솔현저증강6.04%、12.50%화20.33%(P<0.01)，사세포막Cx32적형광강도현저증강23.66%、46.09%화92.18%(P<0.01)。결론：EA가통과증강포막Cx32적표체，증강세포GJ통신공능。
Objective:To investigate the effects of ellagic acid( EA) on the intercellular communication of gap junction( GJ) in Hela cells with stable expresson of Cx32. Methods:The effects of EA on the GJ function were observed by cell labelling fluorescent tracer method. The effects of EA on the Cx32 expression on the membrane of Hela cells were detected by immunofluorescence assay. Results:The cells were treated with 4,8 and 16 μmol/L of EA,the penetration rates of cell fluorescence increased by 6. 04%,12. 50% and 20.33%,respectively(P <0. 01),the fluorescence intensity of the Cx32 on the membrane increased by 23. 66%,46. 09% and 92. 18%,respectively(P<0. 01). Conclusions:EA can enhance the communication function of GJ in Hela cells by promoting the Cx32 on the membrane expression.
