CAJ | 학술논문

目的：观察褪黑素对脑出血大鼠神经功能缺损及血肿周围组织基质金属蛋白酶（MMP）表达的影响。方法将SD雄性大鼠85只，随机分为正常组10只、假手术组25只、模型组25只、褪黑素组25只；后2组建立脑出血模型，后3组随机于12 h、1、2、4和7 d处理，每个时间点5只。修正神经功能缺损评分（NSS）对各组大鼠进行神经功能评估；透射电子显微镜观察出血侧皮质微血管形态；RT-PCR技术定量分析脑组织MMP-2、MMP-9 mRNA表达。结果褪黑素组1～7 d NSS低于模型组（P＜0．05）；褪黑素组和模型组12 h～4 d MMP-2 mRNA水平均逐渐升高，褪黑素组7 d MMP-2 mRNA明显低于模型组（P＜0．05）；模型组MMP-9 mRNA 2 d显著升高，褪黑素组MMP-9 mRNA峰值1 d出现，7 d再次升高，并明显高于模型组（P＜0．05）。结论褪黑素能够改善脑出血大鼠神经功能，对脑出血具有保护作用，其机制可能与褪黑素通过调控MMP-2和MMP-9转录，减轻血管源性脑水肿相关。
목적：관찰퇴흑소대뇌출혈대서신경공능결손급혈종주위조직기질금속단백매（MMP）표체적영향。방법장SD웅성대서85지，수궤분위정상조10지、가수술조25지、모형조25지、퇴흑소조25지；후2조건립뇌출혈모형，후3조수궤우12 h、1、2、4화7 d처리，매개시간점5지。수정신경공능결손평분（NSS）대각조대서진행신경공능평고；투사전자현미경관찰출혈측피질미혈관형태；RT-PCR기술정량분석뇌조직MMP-2、MMP-9 mRNA표체。결과퇴흑소조1～7 d NSS저우모형조（P＜0．05）；퇴흑소조화모형조12 h～4 d MMP-2 mRNA수평균축점승고，퇴흑소조7 d MMP-2 mRNA명현저우모형조（P＜0．05）；모형조MMP-9 mRNA 2 d현저승고，퇴흑소조MMP-9 mRNA봉치1 d출현，7 d재차승고，병명현고우모형조（P＜0．05）。결론퇴흑소능구개선뇌출혈대서신경공능，대뇌출혈구유보호작용，기궤제가능여퇴흑소통과조공MMP-2화MMP-9전록，감경혈관원성뇌수종상관。
Objective To observe the effect of melatonin (MT) on neurological function and MMP2/9 expression in rats following intracerebral hemorrhage (ICH) .Methods Eighty-five SD rats were randomly divided into normal group (n=10) ,sham operation group(n=25) ,ICH model group (n= 25) and MTintervention group (n= 25) .Rats in ICH model group ,sham operation group and MTintervention group were divided into 12h group ,1d group ,2d group ,4d group and 7d group (5 in each group) .Their neurological function was assessed according to the modi-fied NSS .Morphology of microvascular structure was observed under electron microscope .Ex-pression of MMP2/9 mRNA was detected by RT-PCR .Results The NSS score was significantly lower in MTintervention group than in model group on days 1-7 after ICH (P<0 .05) .The ex-pression level of MMP2 mRNA was significantly higher in MT intervention group than in model group from 12h to 4d after ICH and significantly lower on day 7 after ICH (P<0 .05) .The ex-pression level of MMP9 mRNA increased significantly in model group on day 2 after ICH and reached its peak in MT intervention group on day 1 after ICH .The expression level of MMP9 mRNA was significantly higher in MTintervention group than in model group on day 7 after ICH (P<0 .05) .Conclusion MTimproves the neurological function in rats following ICH by regula-ting the MMP2/9 expression and alleviating the vasogenic brain edema .