中华老年心脑血管病杂志
中華老年心腦血管病雜誌
중화노년심뇌혈관병잡지
CHINESE JOURNAL OF GERIATRIC CARDIOVASCULAR AND CEREBROVASCULAR DISEASES
2014年
6期
630-632
,共3页
胡保奎%牛丽丽%耿召华%刘玉峰
鬍保奎%牛麗麗%耿召華%劉玉峰
호보규%우려려%경소화%류옥봉
心肌病 ,肥厚性%肌细胞 ,心脏%RNA ,信使%单体GTP结合蛋白质类
心肌病 ,肥厚性%肌細胞 ,心髒%RNA ,信使%單體GTP結閤蛋白質類
심기병 ,비후성%기세포 ,심장%RNA ,신사%단체GTP결합단백질류
cardiomyopathy,hypertrophic%myocytes,cardiac%RNA,messenger%monomeric GTP-binding proteins
目的：研究Rad基因对心肌细胞肥大的影响，为心肌肥大早期干预提供理论基础。方法针对Rad基因序列构建Rad过表达和低表达腺病毒载体，体外培养乳鼠心肌细胞，分为8组：空白对照组，Ad-GFP组，Ad-RNA干扰（RNAi）组，Ad-Rad组，肥大模型组（心肌营养素1诱导），肥大＋Ad-GFP组，肥大＋ Ad-RNAi组，肥大＋ Ad-Rad组，48 h后检测Rad、心房利钠因子（ANF）mRNA和蛋白表达水平。结果肥大模型组ANF蛋白表达较空白对照组明显升高；肥大＋Ad-Rad组ANF蛋白表达较肥大＋Ad-GFP组明显降低，肥大＋Ad-RNAi组ANF蛋白表达较肥大＋Ad-GFP组明显升高（P＜0．05）。肥大模型组Rad mRNA和蛋白表达较空白对照组明显降低；Ad-Rad组Rad mRNA和蛋白表达较Ad-GFP组明显增加，Ad-RNAi组 Rad mRNA和蛋白表达较Ad-GFP组明显降低（P＜0.05）。结论 Rad基因可抑制在细胞水平心肌营养素1诱导的心肌细胞肥大反应。
目的：研究Rad基因對心肌細胞肥大的影響，為心肌肥大早期榦預提供理論基礎。方法針對Rad基因序列構建Rad過錶達和低錶達腺病毒載體，體外培養乳鼠心肌細胞，分為8組：空白對照組，Ad-GFP組，Ad-RNA榦擾（RNAi）組，Ad-Rad組，肥大模型組（心肌營養素1誘導），肥大＋Ad-GFP組，肥大＋ Ad-RNAi組，肥大＋ Ad-Rad組，48 h後檢測Rad、心房利鈉因子（ANF）mRNA和蛋白錶達水平。結果肥大模型組ANF蛋白錶達較空白對照組明顯升高；肥大＋Ad-Rad組ANF蛋白錶達較肥大＋Ad-GFP組明顯降低，肥大＋Ad-RNAi組ANF蛋白錶達較肥大＋Ad-GFP組明顯升高（P＜0．05）。肥大模型組Rad mRNA和蛋白錶達較空白對照組明顯降低；Ad-Rad組Rad mRNA和蛋白錶達較Ad-GFP組明顯增加，Ad-RNAi組 Rad mRNA和蛋白錶達較Ad-GFP組明顯降低（P＜0.05）。結論 Rad基因可抑製在細胞水平心肌營養素1誘導的心肌細胞肥大反應。
목적：연구Rad기인대심기세포비대적영향，위심기비대조기간예제공이론기출。방법침대Rad기인서렬구건Rad과표체화저표체선병독재체，체외배양유서심기세포，분위8조：공백대조조，Ad-GFP조，Ad-RNA간우（RNAi）조，Ad-Rad조，비대모형조（심기영양소1유도），비대＋Ad-GFP조，비대＋ Ad-RNAi조，비대＋ Ad-Rad조，48 h후검측Rad、심방리납인자（ANF）mRNA화단백표체수평。결과비대모형조ANF단백표체교공백대조조명현승고；비대＋Ad-Rad조ANF단백표체교비대＋Ad-GFP조명현강저，비대＋Ad-RNAi조ANF단백표체교비대＋Ad-GFP조명현승고（P＜0．05）。비대모형조Rad mRNA화단백표체교공백대조조명현강저；Ad-Rad조Rad mRNA화단백표체교Ad-GFP조명현증가，Ad-RNAi조 Rad mRNA화단백표체교Ad-GFP조명현강저（P＜0.05）。결론 Rad기인가억제재세포수평심기영양소1유도적심기세포비대반응。
Objective To provide the theoretical evidence for early treatment of myocardial hyper-trophy by studying the effect of Rad gene on myocardial hypertrophy .Methods Adenovirus vec-tors were constructed for over and low expression of Rad .In vitro cultured suckling rat myocardi-al cells were divided into blank control group ,Ad-GFP group ,Ad-RNAi group ,Ad-Rad group ,hy-pertrophy model group (induced by cardiotrophin 1) ,hypertrophy+ Ad-GFP group ,hypertrophy+ Ad-RNAi group ,hypertrophy+ Ad-Rad group .Expressions of Rad and ANF mRNA and pro-tein were detected 48 h after transfection .Results The expression level of ANF protein was sig-nificantly higher in hypertrophy model group than in blank control group ,significantly lower in hypertrophy+Ad-Rad group than in hypertrophy+Ad-GFP group and significantly higher in hy-pertrophy+ Ad-RNAi group than in hypertrophy + Ad-GFP group (P< 0 .05) .The expression level of Rad mRNA and protein was significantly lower in hypertrophy model group than in blank control group ,significantly higher in hypertrophy+Ad-Rad group than in hypertrophy+Ad-GFP group and significantly lower in hypertrophy + Ad-RNAi group than in hypertrophy + Ad-GFP group (P<0 .05) .Conclusion Rad gene can inhibit cardiotrophin 1-induced myocardial hypertro-phy at cellular level .
