CAJ | 학술논문

目的：观察激动β3肾上腺素受体（β3-AR）对载脂蛋白E基因敲除（apoE-/-）老年高脂小鼠血脂、肝脏前蛋白转化酶枯草溶菌素9（PCSK9）和LDL 受体（LDLR）表达水平的影响。方法选择10周龄雄性C57BL/6小鼠10只（A组）。apoE-/-小鼠40只给予高脂饲料饲养至36周龄，随机分为4组，分别为高脂模型组（B组）、阿托伐他汀阳性药物对照组（C组）、β3-A R激动剂小剂量组（D组）、β3-A R激动剂大剂量组（E组），每组10只，共干预12周。采用Beckman CX7全自动生化分析仪，检测血清 TC、TG、VLDL/LDL-C、HDL-C水平，采用Western blot测定肝脏PCSK9和LDLR表达水平。结果与B组比较，C组、D组和E组 TC、VLDL/LDL-C明显下降，C组、B组、D组LDLR水平明显上调，C组PCSK9水平明显上调（P＜0．05，P＜0．01）；与C组比较，D组和E组TG、PCSK9水平明显下降，D组LDLR水平明显下降，HDL-C水平明显升高，差异有统计学意义（P＜0．05，P＜0．01）。结论β3-AR调脂、升高LDLR、降低PCSK9表达水平是其抗动脉粥样硬化的多重作用机制之一。
목적：관찰격동β3신상선소수체（β3-AR）대재지단백E기인고제（apoE-/-）노년고지소서혈지、간장전단백전화매고초용균소9（PCSK9）화LDL 수체（LDLR）표체수평적영향。방법선택10주령웅성C57BL/6소서10지（A조）。apoE-/-소서40지급여고지사료사양지36주령，수궤분위4조，분별위고지모형조（B조）、아탁벌타정양성약물대조조（C조）、β3-A R격동제소제량조（D조）、β3-A R격동제대제량조（E조），매조10지，공간예12주。채용Beckman CX7전자동생화분석의，검측혈청 TC、TG、VLDL/LDL-C、HDL-C수평，채용Western blot측정간장PCSK9화LDLR표체수평。결과여B조비교，C조、D조화E조 TC、VLDL/LDL-C명현하강，C조、B조、D조LDLR수평명현상조，C조PCSK9수평명현상조（P＜0．05，P＜0．01）；여C조비교，D조화E조TG、PCSK9수평명현하강，D조LDLR수평명현하강，HDL-C수평명현승고，차이유통계학의의（P＜0．05，P＜0．01）。결론β3-AR조지、승고LDLR、강저PCSK9표체수평시기항동맥죽양경화적다중작용궤제지일。
Objective To study the effect of β3-AR agonists on expression of PCSK9 and LDLR in elderly apoE gene knock-out mice .Methods Ten male C57BL/6 mice aged 10 weeks served as group A and 40 apoE gene knock-out mice fed with high fat diet to 36 weeks old were randomly divided into high fat model group (group B) ,positive atrovastatin control group (group C) ,small doseβ3-AR agonist group (group D) and large dose β3-AR agonist group (group E) ,10 in each group .The animals were treated with atorvastatin and β3-AR agonists for 12 weeks .Serum levels of TC ,TG ,VLDL/LDL-C ,HDL-C were measured with the Beckman CX7 automatic biochemical analyzer and expression of PCSK9 and LDLR was detected by Western blot .Results The serum TC and VLDL/LDL-C levels were significantly lower in groups C ,D and E than in group B ,the expression level of LDLR was significantly higher in groups C ,B and D than in group E while that of PCSK9 was significantly higher in group C than in groups B ,D and E (P<0 .05 ,P<0 .01) .The serum TG level was significantly lower in groups D and E than in group C ,the expression level of LDLR was significantly lower while the serum HDL-C level was significantly higher in group D than in group C (P<0 .05 ,P<0 .01) .Conclusion β3-AR agonist decreases TC ,VLDL/LDL-C , TG levels and expression of PCSK9 ,and increases LDLR and HDL-C level ,which are one of its anti-atherosclerosis mechanisms in elderly apoE-/-mice .